|Application ||WB, IHC-F, IF, EM|
|Purification||Antisera to rat TGN38 were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography.|
|Immunogen||Recombinant fusion protein corresponding to extracellular domain of TGN38.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Trans-Golgi network integral membrane protein TGN38, Ttgn1|
|Target/Specificity||Sheep anti-Rat TGN38 antibody recognizes rat TGN38, a 357 amino acid single pass trans membrane glycoprotein found primarily in the Trans-golgi network, and acts as an excellent marker for this cellular organelle (Humphreyet al.1993).TGN38 is likely to have a role in intracellular transport (McNamaraet al.2004) and plays a role in morphological maintenance (Girotti and Banting 1996). It is the homologue of human TGN46 and macaque TGN47 (Ponnambalamet al.1996).|
|Preservative & Stabilisers||0.09% Sodium Azide; 1% Bovine Serum Albumin;|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Rat TGN38 Antibody (Serum) is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Vo, Y.P. et al. (2004) Recycling of the dense-core vesicle membrane protein phogrin in Min6 beta-cells.Biochem Biophys Res Commun. 324: 1004-10. 2. Prabhu, Y. et al. (2014) Defective Transport of the Obesity Mutant PC1/3 N222D Contributes to Loss of Function.Endocrinology. 155: 2391-401. 3. Ni-Komatsu, L. et al. (2008) Identification of quinolines that inhibit melanogenesis by altering tyrosinase family trafficking. Mol Pharmacol. 74:1576-86. 4. Mathews, P.M. et al. (2002) Alzheimer's disease-related overexpression of the cation-dependent mannose 6-phosphate receptor increases Abeta secretion: role for altered lysosomal hydrolase distribution in beta-amyloidogenesis.J Biol Chem. 277: 5299-307. 5. Phillips, S.E. et al. (2006) Specific and nonspecific membrane-binding determinants cooperate in targeting phosphatidylinositol transfer protein beta-isoform to the mammalian trans-Golgi network.Mol Biol Cell. 17: 2498-512. 6. Waugh, M.G. et al. (2011) Detergent-free isolation and characterization of cholesterol-rich membrane domains from trans-Golgi network vesicles.J Lipid Res. 52: 582-9. 7. Farah,. C.A. et al. (2006) Tau interacts with Golgi membranes and mediates their association with microtubules.Cell Motil Cytoskeleton. 63: 710-24. 8. Hesse, D. et al. (2010) Altered GLUT4 trafficking in adipocytes in the absence of the GTPase Arfrp1.Biochem Biophys Res Commun. 394: 896-903. 9. Miranda-Saksena, M. et al. (2002) In rat dorsal root ganglion neurons, herpes simplex virus type 1 tegument forms in the cytoplasm of the cell body.J Virol. 76: 9934-51. 10. Procino, G. et al. (2006) Adipocytes support cAMP-dependent translocation of aquaporin-2 from intracellular sites distinct from the insulin-responsive GLUT4 storage compartment.Am J Physiol Renal Physiol. 290: F985-94. 11. Han, L. et al. (2008) A large form of secretogranin III functions as a sorting receptor for chromogranin A aggregates in PC12 cells.Mol Endocrinol. 22: 1935-49. 12. Probst, O.C. et al. (2006) The 46-kDa mannose 6-phosphate receptor does not depend on endosomal acidification for delivery of hydrolases to lysosomes.J Cell Sci. 2006 Dec 119: 4935-43. 13. Wasmeier, C. et al. (2006) Rab38 and Rab32 control post-Golgi trafficking of melanogenic enzymes.J Cell Biol. 175: 271-81. 14. Hou, J.C. et al. (2006) A specific dileucine motif is required for the GGA-dependent entry of newly synthesized insulin-responsive aminopeptidase into the insulin-responsive compartment.J Biol Chem. 281: 33457-66. 15. Fortin, M.E. et al. (2006) Modulation of GJA1 turnover and intercellular communication by proinflammatory cytokines in the anterior pituitary folliculostellate cell line TtT/GF.Biol Reprod. 74: 2-12. 16. Vasile, E. et al. (2006) IntraGolgi distribution of the Conserved Oligomeric Golgi (COG) complex.Exp Cell Res. 312: 3132-41. 17. Acquatella-Tran Van Ba, I. et al. (2011) Reg-1a is a new neuronal secreted factor that stimulates neurite outgrowth via the Exostosin tumor-like 3 (EXTL3) receptor.J Biol Chem. 287: 4726-39. 18. Watanabe, T. et al. (2012) A unique ball-shaped Golgi apparatus in the rat pituitary gonadotrope: its functional implications in relation to the arrangement of the microtubule network.J Histochem Cytochem. 60: 588-602.2. Luzio, J.P. et al. (1990) Identification, sequencing and expression of an integral membrane protein of the trans-Golgi network (TGN38).Biochem J. 270: 97-102.
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