|Application ||IHC-F, IF, FC|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||Adherent F1 (CBAxBL) bone marrow stormal cells.|
|Shelf Life||18 months from date of despatch.|
|Target/Specificity||Rat anti-Mouse ER-HR3:Biotin, clone ER-HR3 recognizes the murine antigen ER-HR3. ER-HR3 is a cell surface antigen which is expressed by Langerhans cells in epithelium, a subset of mature macrophages and dendritic cells that are located predominantly in haematopoietic and lymphoid organs. Initial studies of ER-HR3 demonstrated expression on peripheral blood monocytes but recent studies suggest that expression is actually very low.During foetal development, ER-HR3 positive cells are localised to haemapoietic islands and are often associated with erythroid progenitor cells. The functions of the ER-HR3 antigen have not been established but reports suggest that the antigen may be involved in adult erythropoiesis and in the regulation of the immune response.Clone ER-HR3 does not inhibit T cell proliferation in antigen-specific T-cell proliferation studies.Clone ER-HR3 recognizes two proteins of approximately 69kD and 55kD under non-reducing conditions.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Mouse ER-HR3 Antibody, clone ER-HR3 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. de Jong, J.P. et al. (1994) A monoclonal antibody (ER-HR3) against murine macrophages. I. Ontogeny, distribution and enzyme histochemical characterization of ER-HR3-positive cells.Cell Tissue Res. 275: 567-76. 2. de Jong, J.P. et al. (1994) A monoclonal antibody (ER-HR3) against murine macrophages.II. Biochemical and functional aspects of the ER-HR3 antigen.Cell Tissue Res. 275:577 – 585. 3. Oliveira, M.A. et al. (2003) Immature macrophages derived from mouse bone marrow produce large amounts of IL-12p40 after LPS stimulation.J Leukoc Biol. 74: 857-67. 4. Throsby, M. et al. (2000) CD11c+ eosinophils in the murine thymus: developmental regulation and recruitment upon MHC class I-restricted thymocyte deletion.J Immunol. 165:1965-75. 5. Grabbe, S. et al. (2002) Beta2 integrins are required for skin homing of primed T cells but not for priming naive T cells.J Clin Invest. 109: 183-92. 6. Sonoda, Y. and Sasaki, K. (2012) Hepatic extramedullary hematopoiesis and macrophages in the adult mouse: histometrical and immunohistochemical studies.Cells Tissues Organs. 196: 555-64. 7. Jacobsen, R.N. et al. (2014) Mobilization with granulocyte colony-stimulating factor blocks medullar erythropoiesis by depleting F4/80+VCAM1+CD169+ER-HR3+Ly6G+ erythroid island macrophages in the mouse.Exp Hematol. pii: S0301-472X(14)00139-8.
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