|Application ||WB, E|
|Calculated MW||22293 Da|
|Immunogen||Recombinant human granulocyte-colony stimulating factor expressed in E. coil.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Granulocyte colony-stimulating factor, G-CSF, Pluripoietin, Filgrastim, Lenograstim, CSF3, C17orf33, GCSF|
|Target/Specificity||Rat anti-Human G-CSF antibody, clone BVD13-3A5 recognizes human granulocyte colony stimulating factor (G-CSF), also known as Pluripoietin. In addition Filgrastim and Lenograstim are used to describe recombinant human G-CSF preparations. Human G-CSF is a 207 amino acid, ~20kDa O-linked glycoprotein belonging to the IL-6 superfamily. There are potentially three isoforms formed by alternative splicing, Rat anti-Human G-CSF antibody, clone BVD13-3A5 binds the canonical isoform-1, binding to other isoforms has not been assessed (UniProt: P09919).G-CSF is produced by activated macrophages, monocytes and neutrophils. In addition it can be produced by fibroblasts and endothelial cells (Zseboet al.1988). G-CSF stimulates production of cells belonging to the granulocytic lineage in the bone marrow and is an activating agent for mature neutrophilic granulocytes (Scapiniet al.2003) to produce other cytokines including CD257 (BAFF), operating through binding to the G-CSF receptor CD114.Rat anti-Human G-CSF antibody, clone BVD13-3A5 has been successfully evaluated as a capture reagent in a highly sensitive sandwich ELISA assay with biotinylated Rat anti-Human G-CSF, clone BVD11-37G10 as a detection reagent and recombinant human G-CSF as antigen.|
|Preservative & Stabilisers||Preservative Free|
|Storage||Store at -20℃ only.|
|Precautions||Anti-Human G-CSF Antibody, clone BVD13-3A5 is for research use only and not for use in diagnostic or therapeutic procedures.|
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