|Application ||WB, IHC-P, IHC-F, E, IP|
|Calculated MW||68479 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein A|
|Immunogen||Purified involucrin from human keratinocytes.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Involucrin, IVL|
|Target/Specificity||Mouse anti-human involucrin antibody, clone SY5 recognizes human involucrin, a 585 amino acid soluble protein encoded by the IVL gene and expressed in epidermal keratinocytes as they tend towards terminal differentiation. involucrin is produced in the stratum spinosum initially in the cell cytosol from where it moves to the cell membrane, associates with transglutaminase and becomes cross-linked to membrane proteins, offering structural support to the cell and forming a stable envelope below the plasma membrane. Mouse anti-human involucrin antibody, clone SY5 recognizes an epitope between residues 421-568 of human involucrin.Involucrin expression is seen in the normal granular cells of the epidermis associated with the plasma membrane during the early phase of cornified envelope maturation (Ishida-Yamamotoet al.1996) and expression is increased in psoriatic epidermis with abnormal cornified envelope maturation (Ishida-Yamamoto and Iizuka 1995). Involucrin staining is also maintained, associated with the cornified envelopein epidermolytic hyperkeratosis, a condition associated with mutations to mutations in genes codong for cytokeratins 1 and 10 (Ishida-Yamamotoet al.1995).|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3)|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human Involucrin Antibody, clone SY5 is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Hudson, D.L. et al. (1992) Characterization of eight monoclonal antibodies to involucrin. Hybridoma . 3: 367-79. 2. Ishida-Yamamoto, A. et al. (1997) Involucrin and SPRR Are Synthesized Sequentially in Differentiating Cultured Epidermal Cells.J Invest Dermatol. 108: 12-6. 3. Caldelari, R. et al. (2000) Long-term culture of murine epidermal keratinocytes.J Invest Dermatol. 114: 1064-5. 4. Ishida-Yamamoto, A. et al. (1996) Immunoelectron microscopic analysis of cornified cell envelope formation in normal and psoriatic epidermis.J Histochem Cytochem. 44: 167-75. 5. Janes,S.M. and Watt, F.M. (2004) Switch from alphavbeta5 to alphavbeta6 integrin expression protects squamous cell carcinomas from anoikis.J Cell Biol. 166: 419-31. 6. Michael, M. et al. (2014) BPAG1-e restricts keratinocyte migration through control of adhesion stability.J Invest Dermatol. 134: 773-82. 7. Hanada, T. et al. (2014) Keratinization induced by air exposure in the reconstructed human epidermal model: An in vitro model of a cultured epithelial autograftJ Biosci Bioeng. pii: S1389-1723(14)00071-1.
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