|Application ||WB, IF, E, Func|
|Calculated MW||77064 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein A|
|Immunogen||Purified porcine transferrin|
|Shelf Life||18 months from date of despatch.|
|Other Names||Serotransferrin, Transferrin, Beta-1 metal-binding globulin, Siderophilin, TF|
|Target/Specificity||anti-transferrin clone HTF-14 is specific for the N-terminal domain of human transferrin and has been shown to block binding of transferrin to the transferrin receptor (CD71). Clone HTF-14 is not able to recognise receptor bound transferrin. Clone HTF-14 recognises a conformational epitope in a loop of the N lobe of transferrin involving the critical Lys-144 residue, also essential for binding of the N lobe to the transferrin receptor (Masonet al.2009)Transferrin is a 77 kDa plasma protein synthesized by the liver involved with the transport of iron. Each transferrin molecule has two domains, both with facility to carry 2 ferric ions. The iron/transferrin complex is essential for haemoglobin synthesis and certain types of cell division.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human Transferrin (N-Terminal) Antibody, clone HTF-14 is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Rubikaite, B.I. et al. (1989) [Identification of the segment for binding of transferrin using a cellular receptor].Mol Biol (Mosk). 23: 765-71. 2. Hradilek, A. and Neuwirt, J. (1986) Iron uptake by MOLT 3 cells from transferrin/monoclonal antitransferrin antibody complexes.Br J Haematol. 62: 21-30. 3. Trebichavsky, I. et al. (1987) Monoclonal antibodies against pig transferrin. Blocking and binding activity.Folia Microbiol (Praha). 32: 448-52. 4. Fuchs, O. et al. (1988) Non-transferrin donors of iron for heme synthesis in immature erythroid cells.Biochim Biophys Acta. 969: 158-65. 5. Bártek, J. et al. (1985) Phylogenetically more conservative epitopes among monoclonal antibody-defined antigenic sites of human transferrin are involved in receptor binding.Br J Haematol. 59: 435-41. 6. Penhallow, R.C. et al. (1986) Comparative studies of the binding and growth-supportive ability of mammalian transferrins in human cells.J Cell Physiol. 128: 251-60. 7. Teh, E.M. et al. (2007) Identification of the epitope of a monoclonal antibody that disrupts binding of human transferrin to the human transferrin receptor.FEBS J. 272: 6344-53. 8. Funk, W.D. et al. (1990) Expression of the Amino-Terminal Half-Molecule of Human Serum Transferrin in Cultured Cells and Characterization of the Recombinant ProteiBiochemistry. 29: 1654-60.
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