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Anti-ATG5-ATG12 Complex (C-Terminal) Antibody

Rabbit Anti-Mouse Polyclonal Antibody

  • WB - Anti-ATG5-ATG12 Complex (C-Terminal) Antibody  ABD10857
    Published customer image:Rabbit anti ATG5-ATG12 Complex antibody used for the evaluation of ATG5-ATG12 conjugate expression in OVCAR-3 cells following induction of autophagy by western blotting.Image caption:The time course of autophagy and apoptosis induction by 30 µM resveratrol treatment. A. Live cell imaging by confocal microscopy (with 10X objective) with Cyto-ID dye in OVCAR-3 cells treated with 30 µM resveratrol at 0, 12, 24 and 48 hour time points. B. Live cell imaging by confocal microscopy (with 10X objective) with caspase 3 green detection dye in OVCAR-3 cells at 0, 12, 24 and 48 hour time points. C. OVCAR-3 cells were treated with 30 µM of resveratrol for 12 h, 24 h, and 48 h. Western blotting of total cell lysates were performed with anti-ATG5-ATG12, LC3, and caspase 3 antibodies. No cleavage of ATG5 was observed. The results shown represent an experiment, in which each time-point of treatment was duplicated.From: Lang F, Qin Z, Li F, Zhang H, Fang Z, Hao E Apoptotic Cell Death Induced by Resveratrol Is Partially Mediated by the Autophagy Pathway in Human Ovarian Cancer Cells. PLoS ONE 10: e0129196.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9CQY1
Other Accession O94817, Q9H1Y0, Q99J83
Reactivity Mouse
Host Rabbit
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 15207 Da
Additional Information
Other Species H
Purification Antiserum to mouse Atg5 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Immunogen KLH conjugated synthetic peptide corresponding to amino acids 262-275 of mouse Atg5. The corresponding sequence differs by one amino acid in human.
Shelf Life 18 months from date of despatch.
Gene ID 67526
Other Names Ubiquitin-like protein ATG12, Autophagy-related protein 12, APG12-like, Atg12, Apg12, Apg12l
Target/Specificity Rabbit anti-ATG5-ATG12 Complex antibody recognizes an epitope within the C-Terminal (CT) region of the Atg5-Atg12 complex, which plays a key role in the regulation of autophagy, and is emerging as an important factor in the innate immune response to viruses (Hwang et al.2012).The Atg5-Atg12 complex interacts with retinoic acid–inducible gene 1 and the adaptor molecule IFN-beta promoter stimulator 1, through caspase recruitment domains, thereby acting as a negative regulator of the type I IFN production pathway, and as a contributor to RNA viral replication within the host cells (Takeshita et al2008).
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-ATG5-ATG12 Complex (C-Terminal) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. Lang, F. et al. (2015) Apoptotic Cell Death Induced by Resveratrol Is Partially Mediated by the Autophagy Pathway in Human Ovarian Cancer Cells.
PLoS One. 10 (6): e0129196.

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Cat# ABD10857
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