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>   home   >   Products   >   Primary Antibodies   >   Anti-14-3-3 beta Antibody (Serum)   

Anti-14-3-3 beta Antibody (Serum)

Rabbit Anti-Sheep Polyclonal Antibody

  • FC - Anti-14-3-3 beta Antibody (Serum) ABD10881
    Published customer image:The expression of 14-3-3 proteins in the vulvar squamous epithelium. Representative samples stained using immunohistochemistry are shown. The expression of the 14-3-3 proteins (β, γ, ζ, ε, η and τ) in normal vulvar squamous epithelium (A–F). The increased cytoplasmic expression of 14-3-3β, γ, ζ, ε and η (G–K) and the reduced nuclear expression of 14-3-3τ (L) in vulvar carcinomas.From: Wang Z, Nesland JM, Suo Z, Trope CG, Holm R The Prognostic Value of 14-3-3 Isoforms in Vulvar Squamous Cell Carcinoma Cases: 14-3-3β and ε Are Independent Prognostic Factors for These Tumors. PLoS ONE 6: e24843.
  • ICC - Anti-14-3-3 beta Antibody (Serum) ABD10881
    Published customer image:Representative immunocytochemistry images of 14-3-3 protein isoforms along and in the zonae pellucidae of cumulus-free oocytes isolated from ovaries of adult mice. The zona-intact cells were fixed in paraformaldehyde but not treated with detergent (see Methods). Paired images of an oocyte (left image is brightfield and right is immunofluorescence) indicate staining along the zona and/or the cell membrane for all isoforms except 14-3-3ζ. Note: the intent of this part of the study was not to examine the intracellular distribution of the isoforms (see Figure 3 for those experiments) since the cells were not permeabilized to permit complete antibody penetration; however, cells may be partially permeabilized by fixation accounting for the detection of intracellular proteins in some cells. (A,B) 14-3-3 β. (C,D) 14-3-3 γ. (E,F) 14-3-3 ε. (G,H) 14-3-3 ζ. (I,J) 14-3-3 η. (K,L) 14-3-3 τ. (M,N) 14-3-3σ. (O,P) Control image without primary antibody addition.From: De S, Marcinkiewicz JL, Vijayaraghavan S, Kline D. Expression of 14-3-3 protein isoforms in mouse oocytes, eggs and ovarian follicular development. BMC Res Notes. 2012 Jan 23;5:57.
  • IF - Anti-14-3-3 beta Antibody (Serum) ABD10881
    Published customer image:Representative immunofluorescence images of 14-3-3 isoforms in oocytes and eggs isolated from adult mice. (A, B) 14-3-3β. (C, D) 14-3-3γ. (E, F) 14-3-3ε. (G, H) 14-3-3ζ. (I, J) 14-3-3 τ. (K, L) 14-3-3&sigma. (M, N) 14-3-3η. Confocal sections with regions of red fluorescence indicating the corresponding isoforms studied (see Methods). The inset in N shows the same egg labeled blue with Hoechst DNA stain (non-confocal image) and confirms that the darker areas in this region of the larger image are condensed metaphase II chromosomes. Control cells were included for each isoform experiment and were imaged using the same confocal settings. Representative control oocytes and eggs are shown in bright-field (O) and fluorescence (P). 14-3-3η accumulates, in part, in the meiotic spindle in eggs as shown by simultaneous labeling with 14-3-3η (Q) and tubulin (R) antibodies. These sequential scans are merged (14-3-3η + tubulin) in (S). The scale bars represent 10 µm for all images.From: De S, Marcinkiewicz JL, Vijayaraghavan S, Kline D. Expression of 14-3-3 protein isoforms in mouse oocytes, eggs and ovarian follicular development. BMC Res Notes. 2012 Jan 23;5:57.
  • WB - Anti-14-3-3 beta Antibody (Serum) ABD10881
    Published customer image:Immunoblots identifying 14-3-3 isoforms in extracts from mouse oocytes, eggs and ovaries. Proteins from cell lysates were separated by electrophoresis under reducing conditions, transferred to membranes and probed with antibodies directed against the 14-3-3 isoforms indicated. Each isoform shown was detected in lysates of 200 oocytes or 200 eggs. Protein extracts of ovaries and brain from adult mice are included for comparison. The 14-3-3 protein is approximately 30 kDa.From: De S, Marcinkiewicz JL, Vijayaraghavan S, Kline D. Expression of 14-3-3 protein isoforms in mouse oocytes, eggs and ovarian follicular development. BMC Res Notes. 2012 Jan 23;5:57.
  • IHC - Anti-14-3-3 beta Antibody (Serum) ABD10881
    Published customer image:Representative immunohistochemistry images of 14-3-3 β in the different stages of follicular development in ovarian sections. Brown staining represents 14-3-3 β against regions counterstained blue with hematoxylin. (A) Primordial follicle. (B) Primary follicle. (C) Secondary follicle. (D) Early antral follicle. (E) Graafian (advanced antral) follicle. (F) Corpus luteum. White arrows indicate the primordial or primary follicles in (A and B). Note the weaker staining in mural granulosa cells in secondary follicles (C, green arrows), the more intense stain along the zona pellucida of the oocyte (C, yellow arrow), and the more intense staining in cells lining the antral cavity (D and E, red arrows). The scale bars represent 10 µm (A-C) or 100 µm (D-F).From: De S, Marcinkiewicz JL, Vijayaraghavan S, Kline D. Expression of 14-3-3 protein isoforms in mouse oocytes, eggs and ovarian follicular development. BMC Res Notes. 2012 Jan 23;5:57.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P68251
Reactivity Sheep
Host Rabbit
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 22054 Da
Additional Information
Other Species M,Rb,H,Rat,B
Purification Antisera to sheep 14-3-3 beta were raised by repeated immunisations of rabbit with highly purified antigen.
Immunogen Synthetic peptide corresponding to acetylated N-terminal sequence of sheep 14-3-3 beta.Peptide sequence: Ac.TMDKSELVC
Shelf Life 18 months from the date of despatch.
Other Names 14-3-3 protein beta/alpha, Protein kinase C inhibitor protein 1, KCIP-1, 14-3-3 protein beta/alpha, N-terminally processed, YWHAB
Target/Specificity Rabbit anti-14-3-3 β antibody recognizes the acetylated N-terminal of 14-3-3 β. It is a member of the 14-3-3 family which consists of 30 kDa proteins that are involved in multiple protein kinase signalling pathways, regulation of cell cycle progression, cytoskeletal structure, transcription, intracellular trafficking and targeting. Protein interactions with 14-3-3 show distinct preference for it’s different isoforms and are regulated by phosphorylation of both 14-3-3 and the bound protein.14-3-3 β plays a role in the pathogenesis of systemic lupus erythematosus and is involved in negative regulation of p90 ribosomal S6 kinase activity and tuberous sclerosis tumour suppressor proteins. It also binds IGF-1 receptor, RAF1 and CDC25 phosphatases. Rabbit anti-14-3-3 β antibody may not react with recombinant proteins that are not N-acetylated.
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-14-3-3 beta Antibody (Serum) is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)

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1. Martin, H. et al. (1993) Antibodies against major brain isoforms of 14-3-3 protein. An antibody specific for the N-acetylated amino-terminus of a protein.
FEBS Lett. 331: 296-303. 2. Chen, H.K. et al. (2003) Interaction of Akt-phosphorylated ataxin-1 with 14-3-3 mediates neurodegeneration in spinocerebellar ataxia type 1.
Cell. 113: 457-68. 3. Wang, Z. et al. (2011) The prognostic value of 14-3-3 isoforms in vulvar squamous cell carcinoma cases: 14-3-3ß and e are independent prognostic factors for these tumors.
PLoS One. 6: e24843. 4. Baxter, H.C. et al. (2002) Immunolocalisation of 14-3-3 isoforms in normal and scrapie-infected murine brain.
Neuroscience. 109: 5-14. 5. Chaudhri, M. et al. (2003) Mammalian and yeast 14-3-3 isoforms form distinct patterns of dimers in vivo.
Biochem Biophys Res Commun. 300: 679-85. 6. Taurino, F. et al. (2014) Short-term Type-1 diabetes differentially modulates 14-3-3 proteins in rat brain and liver.
Eur J Clin Invest. 44: 350-8.1. Aitken, A. et al. (2006) 14-3-3 proteins: A historic overview.
Seminars in Cancer Biology 16: 162-172.

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Cat# ABD10881
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