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Anti-14-3-3 (pSer185) Antibody

Sheep Anti-Sheep Polyclonal Antibody

  • WB - Anti-14-3-3 (pSer185) Antibody  ABD10886
    Western blot with Sheep anti-14-3-3 antibody Lane 1, 2ug recombinant 14-3-3 betaLane 2, 2ug recombinant 14-3-3 zeta Lane 3, sheep brain homogenate, which contains ca. 50% of beta and zeta isoforms that are phosphorylated on Ser 185The recombinant 14-3-3s are expressed in E.coli and are not phosphorylated
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P62262
Other Accession P68253, P29361, P68251, O77642
Reactivity Sheep
Host Sheep
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 29174 Da
Additional Information
Other Species H,B,M,Rat,Rb
Purification Antisera to Phosphorylated 14-3-3 were raised by repeated immunisations of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography.
Immunogen Synthetic peptide corresponding to 14-3-3 beta and zeta phosphorylated at serine 185.Peptide sequence: EILNSPEKAC
Shelf Life 18 months from the date of despatch.
Other Names 14-3-3 protein epsilon, 14-3-3E, Protein kinase C inhibitor protein 1, KCIP-1, YWHAE
Target/Specificity Sheep anti-phosphorylated 14-3-3 antibody recognises the 14-3-3 isoforms beta and zeta which are phosphorylated at serine 185. The members of the 14-3-3 family of proteins are highly conserved and are involved in multiple signalling pathways. The phosphorylated forms of beta and zeta 14-3-3 are found endogenously in brain tissue.
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-14-3-3 (pSer185) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)

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1. Aitken, A. et al. (1995) 14-3-3 alpha and delta are the phosphorylated forms of Raf-activating 14-3-3 beta and zeta. In vivo stoichiometric phosphorylation in brain at Ser-Pro-Glu-Lys Motif.
J. Biol. Chem. 270: 5706 – 5709.

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Cat# ABD10886
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