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>   home   >   Products   >   Primary Antibodies   >   Anti-14-3-3 zeta/delta Antibody (Serum)   

Anti-14-3-3 zeta/delta Antibody (Serum)

Rabbit Anti-Sheep Polyclonal Antibody

  • E - Anti-14-3-3 zeta/delta Antibody (Serum) ABD10888
    Published customer image:Immunoblots identifying 14-3-3 isoforms in extracts from mouse oocytes, eggs and ovaries. Proteins from cell lysates were separated by electrophoresis under reducing conditions, transferred to membranes and probed with antibodies directed against the 14-3-3 isoforms indicated. Each isoform shown was detected in lysates of 200 oocytes or 200 eggs. Protein extracts of ovaries and brain from adult mice are included for comparison. The 14-3-3 protein is approximately 30 kDa.From: De S, Marcinkiewicz JL, Vijayaraghavan S, Kline D. Expression of 14-3-3 protein isoforms in mouse oocytes, eggs and ovarian follicular development. BMC Res Notes. 2012 Jan 23;5:57.
  • ICC - Anti-14-3-3 zeta/delta Antibody (Serum) ABD10888
    Published customer image:Representative immunocytochemistry images of 14-3-3 protein isoforms along and in the zonae pellucidae of cumulus-free oocytes isolated from ovaries of adult mice. The zona-intact cells were fixed in paraformaldehyde but not treated with detergent (see Methods). Paired images of an oocyte (left image is brightfield and right is immunofluorescence) indicate staining along the zona and/or the cell membrane for all isoforms except 14-3-3ζ. Note: the intent of this part of the study was not to examine the intracellular distribution of the isoforms (see Figure 3 for those experiments) since the cells were not permeabilized to permit complete antibody penetration; however, cells may be partially permeabilized by fixation accounting for the detection of intracellular proteins in some cells. (A,B) 14-3-3 β. (C,D) 14-3-3 γ. (E,F) 14-3-3 ε. (G,H) 14-3-3 ζ. (I,J) 14-3-3 η. (K,L) 14-3-3 &tau. (M,N) 14-3-3σ. (O,P) Control image without primary antibody addition.From: De S, Marcinkiewicz JL, Vijayaraghavan S, Kline D. Expression of 14-3-3 protein isoforms in mouse oocytes, eggs and ovarian follicular development. BMC Res Notes. 2012 Jan 23;5:57.
  • IF - Anti-14-3-3 zeta/delta Antibody (Serum) ABD10888
    Published customer image:Representative immunofluorescence images of 14-3-3 isoforms in oocytes and eggs isolated from adult mice. (A, B) 14-3-3β. (C, D) 14-3-3γ. (E, F) 14-3-3ε. (G, H) 14-3-3ζ. (I, J) 14-3-3 τ. (K, L) 14-3-3σ. (M, N) 14-3-3η. Confocal sections with regions of red fluorescence indicating the corresponding isoforms studied (see Methods). The inset in N shows the same egg labeled blue with Hoechst DNA stain (non-confocal image) and confirms that the darker areas in this region of the larger image are condensed metaphase II chromosomes. Control cells were included for each isoform experiment and were imaged using the same confocal settings. Representative control oocytes and eggs are shown in bright-field (O) and fluorescence (P). 14-3-3η accumulates, in part, in the meiotic spindle in eggs as shown by simultaneous labeling with 14-3-3η (Q) and tubulin (R) antibodies. These sequential scans are merged (14-3-3η + tubulin) in (S). The scale bars represent 10 µm for all images.From: De S, Marcinkiewicz JL, Vijayaraghavan S, Kline D. Expression of 14-3-3 protein isoforms in mouse oocytes, eggs and ovarian follicular development. BMC Res Notes. 2012 Jan 23;5:57.
  • IHC - Anti-14-3-3 zeta/delta Antibody (Serum) ABD10888
    Published customer image:Representative immunohistochemistry images of 14-3-3 ζ in the different stages of follicular development in ovarian sections. (A) Primordial follicle. (B) Primary follicle. (C) Secondary follicle. (D) Early antral follicle. (E) Graafian (advanced antral) follicle. (F) Corpus luteum. White arrows indicate the primordial or primary follicles in (A and B). Note the more intense staining in cells lining the antral cavity (E, red arrows). The scale bars represent 10 µm (A-C) or 100 µm (D-F).From: De S, Marcinkiewicz JL, Vijayaraghavan S, Kline D. Expression of 14-3-3 protein isoforms in mouse oocytes, eggs and ovarian follicular development. BMC Res Notes. 2012 Jan 23;5:57.
  • IHC - Anti-14-3-3 zeta/delta Antibody (Serum) ABD10888
    Published customer image:The expression of 14-3-3 proteins in the vulvar squamous epithelium. Representative samples stained using immunohistochemistry are shown. The expression of the 14-3-3 proteins (β, γ, ζ, ε, η and τ) in normal vulvar squamous epithelium (A–F). The increased cytoplasmic expression of 14-3-3β, γ, ζ, ε and η (G–K) and the reduced nuclear expression of 14-3-3τ (L) in vulvar carcinomas.From: Wang Z, Nesland JM, Suo Z, Trope CG, Holm R The Prognostic Value of 14-3-3 Isoforms in Vulvar Squamous Cell Carcinoma Cases: 14-3-3β and ε Are Independent Prognostic Factors for These Tumors. PLoS ONE 6(9): e24843.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P29361
Reactivity Sheep
Host Rabbit
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 27856 Da
Additional Information
Other Species B,C,Rat,H,M,Rb
Purification Antisera to anti 14-3-3 zeta/delta were raised by repeated immunisations of rabbits with highly purified antigen.
Immunogen Synthetic peptide corresponding to acetylated N-terminal sequence of sheep 14-3-3 zeta/delta.Peptide sequence: Ac.MDKNELVQKAC
Shelf Life 18 months from date of despatch.
Other Names 14-3-3 protein zeta/delta, Protein kinase C inhibitor protein 1, KCIP-1, YWHAZ
Target/Specificity Rabbit anti-14-3-3ζ antibody recognizes the acetylated N-terminal of 14-3-3 zeta and the phosphorylated zeta isoform (also known as 14-3-3 delta) in all mammals. These are members of the 14-3-3 family which consists of 30 kDa proteins involved in multiple protein kinase signaling pathways, regulation of cell cycle progression, cytoskeletal structure, transcription, intracellular trafficking and targeting. Protein interactions with 14-3-3 show distinct preference for its different isotypes and are regulated by phosphorylation of both 14-3-3 and the bound protein. 14-3-3ζ is a susceptibility gene for paranoid schizophrenia and is overexpressed in the temporal cortex of cognitively impared Alzheimers patients. The protein binds yeast Sps1/Ste20-related kinase 1 (YSK1) and localizes to the golgi, possibly linking YSK1 signaling, protein transport, cell adhesion and migration. It co-localizes with epidermal growth factor receptor to the plasma membrane following epidermal growth factor signaling. The zeta isoform is involved in MAPKAPK2-mediated phosphorylation which may have a role in p38 MAPK-dependent inflammation. When bound to ADAM 22, 14-3-3 zeta is involved in cell adhesion. 14-3-3ζ also interacts with cofilin, LIM-domain-containing protein kinase 1, protein kinase B/Akt and GPI alpha. 14-3-3ζ can be phosphorylated at serine 184 to produce 14-3-3 delta.Rabbit anti-14-3-3ζ antibody may not react with recombinant proteins that are not N-acetylated.
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-14-3-3 zeta/delta Antibody (Serum) is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)

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1. Martin, H. et al. (1993) Antibodies against the major brain isoforms of 14-3-3 protein. An antibody specific for the N-acetylated amino-terminus of a protein.
FEBS Lett. 331 (3): 296-303. 2. Wang, Z. et al. (2011) The prognostic value of 14-3-3 isoforms in vulvar squamous cell carcinoma cases: 14-3-3β and ε are independent prognostic factors for these tumors.
PLoS One. 6: e24843. 3. Piesiewicz, A. et al. (2012) Pineal arylalkylamine N-acetyltransferase (Aanat) gene expression as a target of inflammatory mediators in the chicken.
Gen Comp Endocrinol. 179 (2): 143-51. 4. Taurino, F. et al. (2014) Short-term type-1 diabetes differentially modulates 14-3-3 proteins in rat brain and liver.
Eur J Clin Invest. 44 (4): 350-8. 5. De, S. et al. (2012) Expression of 14-3-3 protein isoforms in mouse oocytes, eggs and ovarian follicular development
BMC Res Notes. 5: 57.1. Aitken, A. (2006) 14-3-3 proteins: a historic overview.
Semin Cancer Biol. 16 (3): 162-72.

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