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Anti-BAG1 (N-Terminal) Antibody

Rabbit Anti-Human Polyclonal Antibody

  • WB - Anti-BAG1 (N-Terminal) Antibody  ABD10935
    Western blot analysis of whole cell lysate from PC-3 human prostate cancer cells probed with Rabbit anti Human BAG1 at 1(A) and 2(B) ug/ml
  • IHC - Anti-BAG1 (N-Terminal) Antibody  ABD10935
    Immunohistochemical staining of human lymph node with Rabbit anti Human BAG1
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q99933
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 38779 Da
Additional Information
Other Species M
Purification Antisera to BAG1 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Immunogen 14 amino acid peptide near the amino terminus of human BAG1.
Shelf Life 18 months from date of despatch.
Gene ID 573
Other Names BAG family molecular chaperone regulator 1, BAG-1, Bcl-2-associated athanogene 1, BAG1, HAP
Target/Specificity ABD10935 detects an epitope within the N-terminal (NT) region of Bcl-2 associated athanogene-1 (BAG1), a co-chaperone protein which binds to and regulates the 70kD heat shock proteins (Hsp70/Hsc70). BAG1 has also been shown to interact with the kinase Raf1, a number of steroid and tyrosine kinase receptors, and the Bcl-2 family of proteins. It is thought to function as a molecular switch between cell growth and differentiation, and cell survival. TheBAG1 gene encodes three different isoforms; a predominantly cytosolic form of 36kD (BAG1S), a 46kD cytosolic and nuclear form (BAG1M), and a predominantly nuclear form 50kD (BAG1L). BAG1 displays potent neuroprotective activity in vivo against stroke, and could be used for reducing brain injury during cerebral ischemia and neurodegenerative diseases. BAG1 may also promote chemoresistance, growth factor independence, and anchorage independent cell growth in tumour cells.
Preservative & Stabilisers 0.02% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-BAG1 (N-Terminal) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. Liman, J. et al. (2005) Interaction of BAG1 and Hsp70 mediates neuroprotectivity and increases chaperone activity.
Mol. Cell Biol. 25: 3715-3725.1. Gotz, R. et al. (2005) Bag1 is essential for differentiation and survival of hematopoietic and neuronal cells.
Nat. Neurosci. 8: 1169-1178.2. Krajewska, M. et al. (2006) Expression of BAG-1 protein correlates with aggressive behavior of prostate cancers.
Prostate. 66: 801-810.3. Clemo, N.K. et al. (2005) The role of the retinoblastoma protein (Rb) in the nuclear localization of BAG-1: implications for colorectal tumour cell survival.
Biochem. Soc. Trans. 33: 676-678.

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Cat# ABD10935
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