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Anti-PCNA Antibody

Rabbit Anti-Human Polyclonal Antibody

  • IHC - Anti-PCNA Antibody  ABD11034
    Staining of formalin fixed,paraffin embedded human breast with Rabbit anti Human PCNA
  • WB - Anti-PCNA Antibody  ABD11034
    Western blot analysis of PCNA expression in Jurkat (lane 1) and HEK293 (lane 2) whole cell lysates using Rabbit anti Human PCNA polyclonal antibody followed by goat anti Rabbit IgG and visualized by immunofluorescence
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P12004
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 28769 Da
Additional Information
Other Species C,Fish,Rat,Amphibia,B,Pr,M,Dog
Purification Antiserum to human PCNA was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Immunogen Synthetic peptide corresponding an internal region of human PCNA protein.
Shelf Life 18 months from date of despatch.
Gene ID 5111
Other Names Proliferating cell nuclear antigen, PCNA, Cyclin, PCNA
Target/Specificity Rabbit anti-Human PCNA antibody recognizes Human Proliferating Cell Nuclear Antigen (PCNA), also known as cyclin. PCNA is a 261 amino acid ~36kDa nuclear protein vital for cellular DNA synthesis, playing a key role in the DNA damage response where it acts as a loading platform to recruit additional damage repair proteins to lesions following DNA damage and promote repair (Burkovicset al.2009).PCNA is a useful marker for DNA synthesis and is highly conserved among most species. Rabbit anti-human PCNA antibody is expected to have wide species cross reactivity. PCNA expression in human dermis is negatively correlated to age as demonstrated by immunohustochemistry on paraffin sections using rabbit anti-human PCNA antibody (Guninet al.2014).
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-PCNA Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. Gunin, A.G. et al. (2010) Age-Related Changes in Proliferation, the Numbers of Mast Cells, Eosinophils, and cd45-Positive Cells in Human Dermis
J Gerontol A Biol Sci Med Sci. 66: 385-92. 2. Kim, J.Y. et al. (2012) Localization of osteopontin and osterix in periodontal tissue during orthodontic tooth movement in rats.
Angle Orthod. 82: 107-14. 3. Petrov. V.V. et al. (2013) Age-related changes in mast cells and eosinophils of human dermis
Ontogenez. 44: 179-85. 4. Gunin, A.G. et al. (2014) Age-related changes in angiogenesis in human dermis.
Exp Gerontol. 55C: 143-51.

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Cat# ABD11034
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