|Application ||WB, IHC-P|
|Calculated MW||33275 Da|
|Purification||Antisera to Human CD274 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.|
|Immunogen||A peptide corresponding to a 17 amino acid sequence from near the centre of Human CD274.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Programmed cell death 1 ligand 1, PD-L1, PDCD1 ligand 1, Programmed death ligand 1, B7 homolog 1, B7-H1, CD274, CD274, B7H1, PDCD1L1, PDCD1LG1, PDL1|
|Target/Specificity||Rabbit anti-Human CD274 antibody detects CD274, also known as B7-H1 and PD-1L, a cell surface glycoprotein, a member of the B7 family of co-stimulatory molecules. CD274 is expressed constitutively on macrophages and dendritic cells, and is induced on activated T-cells, B-cells, endothelial cells and epithelial cells in response to Interferons alpha, beta and gamma. CD274 is reported to possess dual functions; inhibition of activated effector T cells and co-stimulation of naïve T cells. CD274 inhibits proliferation of activated T cells via ligation to the co-inhibitory molecule CD279 (programmed death-1; PD-1) leading to the secretion of the regulatory cytokine interleukin-10. CD274 has also been shown to co-stimulate early T cell priming and differentiation. Deregulated CD274 function has been reported in chronic viral and intracellular bacterial infection, as well as in many autoimmune diseases and cancers.|
|Preservative & Stabilisers||0.02% Sodium Azide (NaN3)|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human CD274 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. LaGier, A.J. & Pober, J.S. (2006) Immune accessory functions of human endothelial cells are modulated by overexpression of B7-H1 (PDL1). Hum. Immunol. 67: 568-782. Holling TM, et al. (2004) Function and regulation of MHC class II molecules in T-lymphocytes: of mice and men.Hum. Immunol. 65: 282-903. Ishida Y, et al. (1992) Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death. EMBO J. 11: 3887-95.
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