|Application ||IHC-P, E|
|Calculated MW||118173 Da|
|Purification||Antisera to human NALP3 were raised by repeated immunisation of goats with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.|
|Immunogen||Synthetic peptide sequence corresponding to C-EKPELTVVFEPSW from the C Terminus of Human NALP3 (NP_004886).|
|Shelf Life||18 months from date of despatch.|
|Other Names||NACHT, LRR and PYD domains-containing protein 3, Angiotensin/vasopressin receptor AII/AVP-like, Caterpiller protein 1.1, CLR1.1, Cold autoinflammatory syndrome 1 protein, Cryopyrin, PYRIN-containing APAF1-like protein 1, NLRP3, C1orf7, CIAS1, NALP3, PYPAF1|
|Target/Specificity||Goat anti-Human NALP3 antibody recognizes human NACHT, LRR and PYD domains-containing protein 3 (NALP3), also known as Angiotensin/vasopressin receptor AII/AVP-like, Caterpiller protein 1.1 or Cryopyrin. NALP3 is a 1036 amino acid ~120kDa cytoplasmic protein belonging to the NLRP family, containing a single DAPIN domain, nine leucine-rich repeats and a single NACHT domain (UniProt: Q96P20) .NALP3 is involved in inflammation and immune responses. NALP3 may function as an activator of NF-kappaB signalling and play a role in the regulation of inflammation and apoptosis (Choi and Ryter 2014). NALP3 is strongly expressed in polymorphonuclear cells, osteoblasts and chondrocytes, NALP3 can also be detected in non-keratinizing epithelia of oropharynx, esophagus and ectocervix and in the urothelial layer of the bladder.Mutations in the NALP3 gene have been associated with familial cold autoinflammatory syndrome (Hoffmanet al.2001), Muckle-Wells syndrome (Dodéet al.2002), chronic infantile neurological cutaneous and articular syndrome (Feldmannet al.2002) and neonatal-onset multisystem inflammatory disease (Aksentijevichet al.2002).Goat anti-Human NALP3 antibody has been used successfuly for the detection of NALP3 in the eyes of rats by immunohistochemistry on formalin fixed, paraffin embedded sections (Liuet al.2014).|
|Preservative & Stabilisers||0.02% Sodium Azide (NaN3); 0.5% Bovine Serum Albumin;|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human NALP3 (C-Terminal) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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1. Liu, R.T. et al. (2014) Vinpocetine inhibits amyloid-beta induced activation of NF-?B, NLRP3 inflammasome and cytokine production in retinal pigment epithelial cells.Exp Eye Res. pii: S0014-4835(14)00185-7.
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