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Anti-Human Erythropoietin Receptor Antibody

Sheep Anti-Human Polyclonal Antibody

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P14753
Other Accession P19235
Reactivity Human
Host Sheep
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 55194 Da
Additional Information
Other Species M
Purification Antiserum to human EPOR was raised by repeated immunisation of sheep with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Immunogen Partial cDNA of the extracellular domain of human EPOR expressed as a GST fusion protein in E. Coli.
Shelf Life 18 months from date of despatch.
Gene ID 13857
Other Names Erythropoietin receptor, EPO-R, Epor
Target/Specificity ABD11162 recognises Erythropoietin Receptor (EPOR), a type I transmembrane protein and member of the type I cytokine receptor family, expressed by erythroid cells and erythroid progenitor cells, which acts as a receptor for erythropoietin (EPO) to mediate erythroblast proliferation and differentiation, and maintain progenitor survival. Binding of EPO to EPOR, results in the dimerization of the EPOR and the triggering of the JAK2/STAT5 signaling cascade, and in some cell types activation of STAT1 and STAT3. Several isoforms of EPOR exist: EPOR-F, EPOR-S and EPOR-T, and a functional EPOR is found on endothelial cells and cardiomyocytes within the cardiovascular system, suggesting a role in cardiac function. ABD11162 has been shown to block Iodine125 labelled erythropoietin ligand binding using erythroleukemia cell line TF-1 with 3000 EPOR/cell in neutralization studies, see Harris, al. for details.ABD11162 is reported as suitable for use in immunocytochemistry.ABD11162 has been reported for use in functional studies. Removal of Sodium Azide is recommended prior to use in functional assays. AbD Serotec recommend the use of EQU003 for this purpose. The recommended concentration for functional assays is 500ug/ml.
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Store at +4℃. DO NOT FREEZE.
PrecautionsAnti-Human Erythropoietin Receptor Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. Harris, al. (1992) Ligand binding properties of the human erythropoietin receptor extracellular domain expressed in Escherichia coli.
J. Biol. Chem. 267: 15205-15209.

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Cat# ABD11162
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