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Anti-Bovine Neurofilament H 200kDa Antibody

Rabbit Anti-Bovine Polyclonal Antibody

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P19246
Other Accession P12036, P16884
Reactivity Bovine
Host Rabbit
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 116994 Da
Additional Information
Other Species Rat,H,M
Purification Antiserum to bovine NFH was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Immunogen GST-tagged bovine NFH recombinant protein.
Shelf Life 18 months from date of despatch.
Gene ID 380684
Other Names Neurofilament heavy polypeptide, NF-H, 200 kDa neurofilament protein, Neurofilament triplet H protein, Nefh, Kiaa0845, Nfh
Target/Specificity Rabbit anti-Bovine Neurofilament H 200kDa antibody recognizes bovine intermediate 200kDa Neurofilament H (NFH), a 1026 amino acid ~200kDa protein involved in the maintenence of neuronal integrity.Neurofilaments are a major component of the cellular cytoskeleton, acting as the most abundant support for the axon cytoplasm. They exist as three neurofilament proteins: 68/70kDa (NFL) 160kDa (NFM) and 200kDa (NFH), which are expressed in both the central and peripheral nervous systems. Although typically expressed by neurons, neurofilaments are also expressed by neuroblastomas, neuromas, gangliogliomas, ganglioneuromas, and ganglioneuroblastomas, as well as paragangliomas, carcinoids, skin neuroendocrine carcinomas, and oat cell carcinomas of the lung (Lehtoet al.1983).Defects in NFH are responsible for susceptibility to the neurodegenerative disorder amyotrophic lateral sclerosis (ALS) which affects upper and lower motor neurons, and results in fatal paralysis (Mendonçaet al.2005).
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Bovine Neurofilament H 200kDa Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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1. Kaneko, S. et al. (2006) Protecting axonal degeneration by increasing nicotinamide adenine dinucleotide levels in experimental autoimmune encephalomyelitis models.
J Neurosci. 26: 9794-804. 2. Li, Y. et al. (2008) Transplanted olfactory ensheathing cells incorporated into the optic nerve head ensheathe retinal ganglion cell axons: possible relevance to glaucoma.
Neurosci Lett. 440: 251-4. 3. Ignatov, A. et al. (2003) Role of the G-protein-coupled receptor GPR12 as high-affinity receptor for sphingosylphosphorylcholine and its expression and function in brain development.
J Neurosci. 23: 907-14. 4. Leypoldt, F. et al. (2002) Neuronal differentiation of cultured human NTERA-2cl.D1 cells leads to increased expression of synapsins.
Neurosci Lett. 2002 May 10;324(1):37-40. 5. Savolainen, H. et al. (2009) Cortical demyelination in PML and MS: similarities and differences.
Neurology. 72: 861. 6. Zhang, Z. et al. (2011) The role of single cell derived vascular resident endothelial progenitor cells in the enhancement of vascularization in scaffold-based skin regeneration.
Biomaterials. 32: 4109-17. 1. Liu, Q. et al. (2004) Neurofilament proteins in neurodegenerative diseases.
Cell. Mol. Life Sci.61: 3057-3075.

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Cat# ABD11268
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