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Anti-NF-Y Antibody

Rabbit Anti-Human Polyclonal Antibody

  • WB - Anti-NF-Y Antibody  ABD11274
    Western blot analysis of NF-Y expression in chromatin lysates of CHO-7 cells, a clone of CHO-K1 cells adapted for growth in lipoprotein deficient medium, grown in the absence (I) or presence (S) of cholesterol probed with Rabbit anti Mouse NF-Y
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P23511
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 36877 Da
Additional Information
Other Species M,Ha
Purification Antisera to NF-Y were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by ion exchange chromatography.
Immunogen NF-Y (A subunit) peptide corresponding to a region near the N-terminus conjugated to Keyhole Limpet Hemocyanin.
Shelf Life 18 months from date of despatch.
Gene ID 4800
Other Names Nuclear transcription factor Y subunit alpha, CAAT box DNA-binding protein subunit A, Nuclear transcription factor Y subunit A, NF-YA, NFYA
Target/Specificity Rabbit anti-NF-Y antibody recognizes human Nuclear transcription factor Y subunit alpha, also known as NF-Y subunit a, NF-YA or CAAT box DNA-binding protein subunit A. Rabbit anti-NF-Y antibody recognizes both differentially spliced 35 kDa and 42 kDa forms of the A subunit of NF-Y but demonstrates no reactivity against the 25 kDa B subunit.
Preservative & Stabilisers 0.01% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-NF-Y Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)

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1. Nenoi, M. et al. (2001) Regulation of the catalase gene promoter by Sp1, CCAAT-recognizing factors, and a WT1/Egr-related factor in hydrogen peroxide-resistant HP100 cells.
Cancer. Res. 61: 5885-94. 2. Paradkar, P.N. and Roth, J.A. (2007) Expression of the 1B isoforms of divalent metal transporter (DMT1) is regulated by interaction of NF-Y with a CCAAT-box element near the transcription start site.
J Cell Physiol. 211: 183-8. 3. Petrie, J.L. et al. (2012) Glucose Induces Protein-Targeting-to-Glycogen in Hepatocytes by Fructose 2,6-bisphosphate mediated recruitment of MondoA to the Promoter.
Mol Cell Biol. 33: 725-. 4. Gromnicova, R. et al. (2012) Transcriptional control of the multi-drug transporter ABCB1 by transcription factor Sp3 in different human tissues.
PLoS One. 7 (10): e48189.

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Cat# ABD11274
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