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Anti-Glutamate Decarboxylase 1/2 Antibody

Rabbit Anti-Rat Polyclonal Antibody

  • E - Anti-Glutamate Decarboxylase 1/2 Antibody  ABD11280
    Published customer image:Rabbit anti glutamate decarboxylase 1/2 antibody used for the evaluation of hGAD65 expression levels in Gad65 expressing algal transformants by sanwich ELISA.Image caption:Expression levels as percentages of hGAD65 in total soluble protein (TSP) of hGAD65 expressing C. reinhardtii transformants. The expression level was determined by hGAD65 specific ELISA as described in detail in methods. hGAD-1 and hGAD-2, two independent algal transformants; WT, untransformed wild type C. reinhardtii 137c strain.From:Wang X, Brandsma M, Tremblay R, Maxwell D, Jevnikar AM, Huner N, Ma S. A novel expression platform for the production of diabetes-associated autoantigen human glutamic acid decarboxylase .BMC Biotechnol. 2008 Nov 17;8:87.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q05683
Reactivity Rat
Host Rabbit
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 65402 Da
Additional Information
Other Species Cat,H,M
Purification Antiserum to glutamic acid decarboxylase was raised by repeated immunisation of with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography on protein A.
Immunogen Synthetic peptide sequence D-F-L-I-E-E-I-E-R-L-G-Q-D-L from the C terminus of rat GAD2
Shelf Life 18 months from date of despatch.
Gene ID 24380
Other Names Glutamate decarboxylase 2,, 65 kDa glutamic acid decarboxylase, GAD-65, Glutamate decarboxylase 65 kDa isoform, Gad2, Gad65
Target/Specificity Rabbit anti-Glutamate Decarboxylase 1/2 antibody recognizes both molecular forms of glutamate decarboxylase, GAD1 (GAD67) and GAD2 (GAD65), the enzyme which converts glutamic acid to gamma -aminobutyric acid (GABA), the major inhibitory transmitter in the higher brain region.
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Glutamate Decarboxylase 1/2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. Hernandez-Montiel, H.L. et al. (2003) Diffusible signals and fasciculated growth in reticulospinal axon pathfinding in the hindbrain.
Dev. Biol. 255: 99-112. 2. Baizer, J.S. et al. (2011) Neurochemical and structural organization of the principal nucleus of the inferior olive in the human.
Anat Rec (Hoboken). 294: 1198-216. 3. Klusa, V. et al. (2013) Mildronate enhances learning/memory and changes hippocampal protein expression in trained rats.
Pharmacol Biochem Behav. pii: S0091-3057(13)00078-6. 4. Wang, X. et al. (2008) A novel expression platform for the production of diabetes-associated autoantigen human glutamic acid decarboxylase (hGAD65).
BMC Biotechnol. 8:87

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Cat# ABD11280
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