|Application ||WB, E, IP, GS|
|Calculated MW||60219 Da|
|Purification||Antisera to human NF-kB were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography.|
|Immunogen||Human p65 (residues 12-317) expressed in E. coli|
|Shelf Life||18 months from date of despatch.|
|Other Names||Transcription factor p65, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, RELA, NFKB3|
|Target/Specificity||Sheep anti-Human NFkB p65 antibody recognizes human full length human p65 (NF-kB), also known as Transcription factor p65 or Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3. Human NFkB p65 is a 551 amino acid plieotropic transcription factor containing a single RHD domain. Sheep anti-Human NFkB p65 antibody will also detect proteolytic fragments of NFkB p65 containing the DNA binding RHD domain located in the amino-terminal region. Multiple isoforms of NFkB p65 are generated by alternative splicing. Sheep anti-Human NFkB p65 antibody binds to isoforms 1 and 2 containing the intact RHD domain, but it is unclear whether it will bind to isoforms 3 and 4 which have partial differing deletions within the RHD domain.|
|Preservative & Stabilisers||0.09% Sodium Azide; 0.5% Bovine Serum Albumin;|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human NFkB p65 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Lee, J.H. et al. (2007) Heparin inhibits NF-κB activationand increases cell death in cerebral endothelial cells after oxygen-glucose deprivation.J Mol Neurosci. 32: 145-54.
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