|Application ||WB, IHC-F|
|Calculated MW||36522 Da|
|Purification||Antisera to DFF were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by ion exchange chromatography.|
|Immunogen||Peptide from C-terminus of human DFF|
|Shelf Life||18 months from date of despatch.|
|Other Names||DNA fragmentation factor subunit alpha, DNA fragmentation factor 45 kDa subunit, DFF-45, Inhibitor of CAD, ICAD, DFFA, DFF1, DFF45|
|Target/Specificity||ABD11307 recognises the 45kD chain of a heterodimeric apoptosis related protein designated DFF (DNA fragmentation factor). DFF-45 is a substrate for Caspase-3, which cleaves the protein at two sites to generate factors that cause DNA fragmentation.ABD11307 recognises both intact DFF-45 and cleaved fragments at the C-terminus.|
|Preservative & Stabilisers||0.02% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human DFF (C-Terminal) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Gabdoulkhakova, A. et al. (2007) High rate of mutation reporter gene inactivation during human T cell proliferation.Immunogenetics. 59: 135-43.1. Liu, X. et al. (1997) DFF, a heterodimeric protein that functions downstream of Caspase-3 to trigger DNA fragmentation during apoptosis.Cell 89: 175-184.
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