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Anti-Amyloid Precursor Protein (C-Terminal) Antibody

  • IHC - Anti-Amyloid Precursor Protein (C-Terminal) Antibody  ABD11339
    Immunohistochemical staining of rat brain with Rabbit anti amyloid precursor protein
  • WB - Anti-Amyloid Precursor Protein (C-Terminal) Antibody  ABD11339
    Published customer image:Rabbit anti Amyloid precursor protein antibody used to detect the amyloid β CT fragment by western blotting. Image caption:Western blotting analysis result of BACE1 and ß-CTF. A: bands of BACE1, ß-CTF and ß-tubulin; B: optical density analysis of BACE1; C: optical density analysis of ß-CTF. *: compared with normal control group: p0.05; ?: compared with normal control group: p
  • WB - Anti-Amyloid Precursor Protein (C-Terminal) Antibody  ABD11339
    Western blot analysis of APP in mouse (M) and rat (R) brain tissue lysates with AHP538
  • WB - Anti-Amyloid Precursor Protein (C-Terminal) Antibody  ABD11339
    Published customer image:Rabbit anti Amyloid precursor protein antibody used for the detection of the amyloid C99 fragment by western blotting of leptomeningeal lysatesImage caption:Characterization of BACE1 elevation in meningeal cells associated with leptomeningeal amyloidosis. Panel (A) shows BACE1 labeled cells aligning along the pia mater in a case with cerebral amyloid angiopathy with prominent leptomeningeal amyloidosis (B), in contrast to a control case wherein no BACE1 and Aß labeling is present at the pia (C,D). Arrows in (A,B) points to profiles with increased BACE1 and 6E10 IR over the background. Panel (E) shows a preparation of leptomeninge sample from the temporal lobe (E), which are rinsed thoroughly in cold phosphate buffer for biochemical analysis (F). Levels of BACE1 protein and C99 in leptomeningeal lysates are elevated in 5 cases with CAA relative to 5 control cases (G,H), as is BACE1 enzymatic activity measured in the lysates (H). Aß42 levels are also higher in the CAA group relative to control (H). In primary cell culture of leptomeningeal biopsies, meningeal cells appear polygonal under phase contrast microscope (J), co-express fibronectin (K) and BACE1 (L). These cells also express the ß-amyloid precursor protein (H, the corresponding FN labeling image is not shown). Scale bar?=?250 µm in (A) applying to (B-D), equivalent to 50 µm for inserts in (A,B), 25 µm for (J) and 12.5 µm for (K-M).From: Xue ZQ, He ZW, Yu JJ, Cai Y, Qiu WY, Pan A, Gai WP, Cai H, Luo XG, Ma C, Yan XX. Non-neuronal and neuronal BACE1 elevation in association with angiopathic and leptomeningeal ß-amyloid deposition in the human brain.BMC Neurol. 2015 May 2;15:71.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P05067
Host Rabbit
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 86943 Da
Additional Information
Other Species H,Rat,M
Purification Antisera to APP were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Immunogen Synthetic peptide corresponding to amino acids 737-751 of human APP.
Shelf Life 18 months from date of despatch.
Gene ID 351
Other Names Amyloid beta A4 protein, ABPP, APPI, APP, Alzheimer disease amyloid protein, Cerebral vascular amyloid peptide, CVAP, PreA4, Protease nexin-II, PN-II, N-APP, Soluble APP-alpha, S-APP-alpha, Soluble APP-beta, S-APP-beta, C99, Beta-amyloid protein 42, Beta-APP42, Beta-amyloid protein 40, Beta-APP40, C83, P3(42), P3(40), C80, Gamma-secretase C-terminal fragment 59, Amyloid intracellular domain 59, AICD-59, AID(59), Gamma-CTF(59), Gamma-secretase C-terminal fragment 57, Amyloid intracellular domain 57, AICD-57, AID(57), Gamma-CTF(57), Gamma-secretase C-terminal fragment 50, Amyloid intracellular domain 50, AICD-50, AID(50), Gamma-CTF(50), C31, APP, A4, AD1
Target/Specificity Rabbit anti-Amyloid Precursor Protein antibody recognizes both intact Amyloid Precursor Protein (APP), and also the C99 fragment generated by Beta-secretase.The sequence recognised by this antibody corresponds to amino acids 85-99 of the C99 fragment. The C99 fragment itself is a substrate for gamma-secretase to generate the 4 kDa beta amyloid peptide, found in the brains of Alzheimer's disease patients. APP also inhibits Notch signaling through it’s interaction with NUMB (Roncaratiet al.2002). Rabbit anti-Amyloid Precursor Protein antibody has been used successfully for the detection of the ~14 kDa β C-terminal fragment of APP, producted as a result of cleavage by BACE1 using western blotting -in rat retinal lysates (Huanget al.2012).
Preservative & Stabilisers 0.02% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Amyloid Precursor Protein (C-Terminal) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. Yan, X.X. et al. (2007) beta-Secretase expression in normal and functionally deprived rat olfactory bulbs: inverse correlation with oxidative metabolic activity.
J Comp Neurol. 501: 52-69. 2. Zhang, H. et al. (2011) IGF-1 reduces BACE-1 expression in PC12 cells via activation of PI3-K/Akt and MAPK/ERK1/2 signaling pathways.
Neurochem Res. 36: 49-57. 3. Désiré, L. et al. (2005) RAC1 inhibition targets amyloid precursor protein processing by gamma-secretase and decreases Abeta production in vitroandin vivo.
J Biol Chem. 280: 37516-25. 4. Huang JF et al. (2012) Timosaponin-BII inhibits the up-regulation of BACE1 induced by ferric chloride in rat retina.
BMC Complement Altern Med. 12: 189. 5. Xiong, K. et al. (2007) Mitochondrial respiratory inhibition and oxidative stress elevate beta-secretase (BACE1) proteins and activity in vivo in the rat retina.
Exp Brain Res. 181: 435-46. 6. Zhang, X.M. et al. (2010) Functional deprivation promotes amyloid plaque pathogenesis in Tg2576 mouse olfactory bulb and piriform cortex.
Eur J Neurosci. 31: 710-21. 7. Cai, Y. et al. (2010) β-Secretase-1 elevation in aged monkey and Alzheimer's disease human cerebral cortex occurs around the vasculature in partnership with multisystem axon terminal pathogenesis and β-amyloid accumulation.
Eur J Neurosci. 32: 1223-38. 8. Marcade, M. et al. (2008) Etazolate, a neuroprotective drug linking GABA(A) receptor pharmacology to amyloid precursor protein processing.
J Neurochem. 106: 392-404. 9. Cai, Y. et al. (2012) BACE1 elevation is involved in amyloid plaque development in the triple transgenic model of Alzheimer's disease: differential Aβ antibody labeling of early-onset axon terminal pathology.
Neurotox Res. 21: 160-74. 10. Zhang, H. et al. (2015) Hydrogen sulfide-induced processing of the amyloid precursor protein in SH-SY5Y human neuroblastoma cells involves the PI3-K/Akt signaling pathway.
Cell Mol Neurobiol. 35 (2): 265-72. 11. Xue, Z.Q. et al. (2015) Non-neuronal and neuronal BACE1 elevation in association with angiopathic and leptomeningeal ß-amyloid deposition in the human brain.
BMC Neurol. 15: 71.1. Ponte, P. et al. (1988) A new A4 amyloid mRNA contains a domain homologous to serine proteinase inhibitors.
Nature 331: 525-527.2. Selkoe, D.J. (1994) Cell biology of the amyloid beta protein precursor and the mechanism of Alzheimer's disease.
Ann. Rev. Cell. Biol. 10: 373-403.

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