|Application ||WB, IF|
|Calculated MW||226666 Da|
|Purification||Antisera to human MDC1 (3838) were raised by repeated immunisations of sheep with highly purified antigen.|
|Immunogen||Recombinant MDC1 BRCT domains (aa 1883 - 2089).|
|Shelf Life||18 months from date of despatch.|
|Other Names||Mediator of DNA damage checkpoint protein 1, Nuclear factor with BRCT domains 1, MDC1, KIAA0170, NFBD1|
|Target/Specificity||Sheep anti-Human MDC1 antibody recognizes human Mediator of DNA damage checkpoint protein 1, also known as MDC1 or Nuclear factor with BRCT domains 1. MDC1 is a 2089 amino acid, ~250 kDa nuclear protein containing two BRCT domains and a single FHA domain. MDC1 is involved in checkpoint mediated cell cycle arest in response to DNA damage (UniProt: Q14676)|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human MDC1 (aa1883-2089) Antibody (Serum) is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Goldberg, M. et al. (2003) MDC1 is required for the intra-S-phase DNA damage checkpoint.Nature. 421: 952 - 956 2. Lou, Z. et al. (2003) MDC1 is coupled to activated CHK2 in mammalian DNA damage response pathways.Nature. 421: 957 - 961 4. Shi, W. et al. (2008) Disassembly of MDC1 foci is controlled by ubiquitin-proteasome-dependentdegradation.J Biol Chem. 283: 31608-16. 5. Rai, R. et al (2008) Differential regulation of centrosome integrity by DNA damage response proteins.Cell Cycle. 7: 2225-33. 6. Ichijima, Y. et al. (2011) MDC1 directs chromosome-wide silencing of the sex chromosomes in male germ cells.Genes Dev. 25: 959-71. 7. Sin, H.S. et al. (2012) Human postmeiotic sex chromatin and its impact on sex chromosome evolution.Genome Res. 22: 827-36. 8. Nakada, S. et al. (2008) PP4 is a gamma H2AX phosphatase required for recovery from the DNA damage checkpoint.EMBO Rep. 9: 1019-26. 9. Nakada, S. et al. (2010) Non-canonical inhibition of DNA damage-dependent ubiquitination by OTUB1.Nature. 466: 941-6. 10. Reichert, S. et al. (2011) Survivin inhibition and DNA double-strand break repair: A molecular mechanism to overcome radioresistance in glioblastomaRadiother Oncol. 101: 51-8.3. Stewart, G.S. et al. (2003) MDC1 is a mediator of the mammalian DNA damage checkpoint.Nature. 421: 961 - 966
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