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Mouse Anti-Human Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| FC |
|---|---|
| Primary Accession | P03989 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Clone Names | HLA-ABC-m3 |
| Calculated MW | 40428 Da |
| Purification | Purified IgG prepared by ion exchange chromatography |
|---|---|
| Immunogen | Immune complex precipitated from HLA-B27 positive cell line (Bordin) by anti HLA-antibody and Staphylococcal Protein A. |
| Shelf Life | 18 months from date of despatch. |
| Other Names | HLA class I histocompatibility antigen, B-27 alpha chain, MHC class I antigen B*27, HLA-B, HLAB |
| Target/Specificity | Mouse anti-Human HLA B27 antibody, clone HLA-ABC-m3 recognizes the HLA-B27 alloantigen. This antibody reacts with the peripheral blood lymphocytes of 47/47 individuals conventionally typed as HLA-B27 + and precipitates cell surface molecules of 43 and 12 kD, corresponding to the HLA Class 1 heavy chain and beta 2 microglobulin.Affinity studies by Scatchard analysis showed that this antibody has a higher affinity for HLA-B27 (9.7 x 108M-1) than for HLA B7 (9.5 x 107M-1).Subjects Expected Fluorescence Intensity Heterozygous HLA-B27+ Strong Homozygous HLA-B27+ Strong Heterozygous HLA-B7+ Faint Non B27, Non B7 Negative |
| Preservative & Stabilisers | 0.09% Sodium Azide (NaN3); 1% Bovine Serum Albumin |
| Storage | Store at +4℃ or -20℃. |
| Precautions | Anti-Human HLA B27 Antibody, clone HLA-ABC-m3 (FITC) is for research use only and not for use in diagnostic or therapeutic procedures. |
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Provided below are standard protocols that you may find useful for product applications.
References
1. Trapani, J.A., et al. (1983) Description of a mouse monoclonal anti HLA-B27 antibody, HLA-ABC-m3 Human Immunology 7: 205-216. 2. Orr, K. et al. (1994) Utilization of commercial antisera and flow cytometry in HLA-B27 typing.Cytometry (Comm. Clin. Cytometry) 18: 17-20. 3. Levering, W.H. et al. (2003) Flow cytometric HLA-B27 screening: cross-reactivity patterns of commercially available anti-HLA-B27 monoclonal antibodies with other HLA-B antigens.Cytometry B Clin Cytom. 54: 28-38. 4. Mandic, R. et al. (2004) Comparison of surface HLA class I levels in squamous cell carcinoma cell lines of the head and neck.Anticancer Res. 24(: 973-9. 5. Goodall, J.C. et al. (2007) Does HLA-B27 influence the monocyte inflammatory response to lipopolysaccharide?Rheumatology (Oxford). 46: 232-7. 6. Meyer, O. et al. (2006) A simple and practical agglutination assay for human leucocyte antigen-B27 typing.Vox Sang. 91 (1): 77-80. 7. Meyer, O. et al. (2008) Application of the particle gel agglutination assay in the typing of single human leucocyte antigens.Tissue Antigens. 71 (2): 157-9. 8. Belaunzaran, M. et al. (2015) HLA-B27-Homodimer-Specific Antibody Modulates the Expansion of Pro-Inflammatory T-Cells in HLA-B27 Transgenic RatsPLOS ONE. 10 (6): e0130811.
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