|Application ||IHC-F, FC, IP|
|Other Species||Rainbow Trout|
|Purification||Purified IgM prepared by gel filtration from tissue culture supernatant|
|Immunogen||Purified CD60 antigen from PBL; 9-O-acetyl-GD3 from buttermilk|
|Shelf Life||18 months from date of despatch.|
|Target/Specificity||Mouse anti-Human CD60 antibody, clone M-T6004 recognizes the human CD60 antigen, a carbohydrate structure GD3 (Neu5Aca8Neu5Aca3Galβ4GlcCer) modified by a terminal 9-0-acetylated disialosyl group (9-O-acetyl GD3). Mouse anti-human CD60 antibody clone M-T6004 does not recognize 7-O-acetyl GD3 or non-acetylated GD3. CD60 has been divided into 3 sub-types depending on the acetylation present. Non acetylated GD3 is termed CD60a and 7-O-acetyl GD3 termed CD60c. 9-O-acetyl GD3 as recognized by clone M-T6004 has been designated CD60b.CD60b is present on CD8+ve helper T cells (Rieber and Rank 1994), CD4+ve memory T cells and on activated B cells (Vateret al.1997). Expression has also been reported on neuroectodermal cells (Carret al.1995) and in breast carcinomas (Gochtet al.1998). CD60b is not expressed by naive T cells (Wadaet al.1998). GD3 has been described as a pro-apoptotic messenger targeting mitochondria in many lymphoid and myeloid cell types (García-Ruizet al.2000). Acetylation abrogates the pro-apoptotic activity of GD3 (Malisanet al.2002) and CD60b protects tumor cells from apoptosis (Kniepet al.2006).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human CD60 Antibody, clone M-T6004 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Rieber, E. P. and Rank, G. (1994) CDw60: a marker for human CD8+ T helper cells.J. Exp. Med. 179: 1385-1390. 2. Saalmuller, A. et al. (2007) Summary of the animal homologue section of HLDA8.Vet Immunol Immunopathol. 119: 2-13. 3. Malisan, F. et al. (2002) Acetylation suppresses the proapoptotic activity of GD3 ganglioside.J Exp Med. 196: 1535-41. 4. Kniep, B. et al. (2006) 9-O-acetyl GD3 protects tumor cells from apoptosis.Int J Cancer. 119: 67-73. 5. Erdmann, M. et al. (2006) Differential surface expression and possible function of 9-O- and 7-O-acetylated GD3 (CD60 b and c) during activation and apoptosis of human tonsillar B and T lymphocytes.Glycoconj J. 23: 627-38. 6. Fischer, U. and Koellner, B. (2007) Cross-reactivity of human leukocyte differentiation antigen monoclonal antibodies on carp and rainbow trout cells.Vet Immunol Immunopathol. 119: 142-55. 7. Mukherjee, K. et al. (2008) O-acetylation of GD3 prevents its apoptotic effect and promotes survival of lymphoblasts in childhood acute lymphoblastic leukaemiaJ Cell Biochem. 105: 724-34.
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