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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Anti-Human Bcl-2 Antibody, clone 100    

Anti-Human Bcl-2 Antibody, clone 100

Mouse Anti-Human Monoclonal Antibody

  • IHC - Anti-Human Bcl-2 Antibody, clone 100  ABD11595
    Staining of paraffin embedded human tonsil with Mouse anti Human Bcl-2
  • WB - Anti-Human Bcl-2 Antibody, clone 100  ABD11595
    Western blot analysis of Jurkat T cell leukemic whole cell lysate probed with Mouse anti Human Bcl-2 antibody followed by HRP conjugated Goat anti Mouse IgG secondary antibody, visualized using chemiluminescence
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P10415
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names 100
Calculated MW 26266 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen Synthetic peptide, amino acids 41-54 of the Bcl-2 protein
Shelf Life 18 months from date of despatch.
Gene ID 596
Other Names Apoptosis regulator Bcl-2, BCL2
Target/Specificity Mouse anti-Human Bcl-2 antibody, clone 100 recognizes the human Apoptosis regulator Bcl-2 oncoprotein, a 239 amino acid ~25kDa integral single pass membrane protein containing 4 BH motifs which lies within the cell rather than on the cell surface. The protein is localised in the outer mitochondrial membrane and plays a role in the inhibition of apoptosis. Mouse anti-Human Bcl-2, clone 100 reacts with small B lymphocytes in the mantle zone and many cells within T cell areas. Very few cells in germinal centres are stained. In the thymus many cells in the medulla are stained but the cortex shows weak or negative staining. In non-haematopoietic tissues few cells are stained (Krajewskiet al.1995). The antibody reacts with neoplastic cells of follicular lymphoma and anaplastic large cell lymphoma (Reed 1997).Mouse anti-human Bcl-2, clone 100 has been successfully used to demonstrate Bcl-2 in formalin fixed, paraffin embedded tissues of radioresistant squamous cell carcinoma by immunohistochemistry (Condonet al.2002).
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Human Bcl-2 Antibody, clone 100 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. Tsujimoto, Y. & Croce, C.M. (1986) Analysis of the structure, transcripts, and protein products of bcl-2, the gene involved in human follicular lymphoma.
Proc Natl Acad Sci U S A. 83 (14): 5214-8. 2. Cleary, M.L. et al. (1986) Cloning and structural analysis of cDNAs for bcl-2 and a hybrid bcl-2/immunoglobulin transcript resulting from the t(14;18) translocation.
Cell. 47: 19-28. 3. Pezzella, F. et al. (1990) Expression of the bcl-2 oncogene protein is not specific for the 14;18 chromosomal translocation.
Am J Pathol. 137 (2): 225-32. 4. Hockenbery, D. et al. (1990) Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death.
Nature. 348 (6299): 334-6. 5. Iannone, F. et al. (2005) Increased Bcl-2/p53 ratio in human osteoarthritic cartilage: a possible role in regulation of chondrocyte metabolism.
Ann Rheum Dis. 64: 217-21. 6. Iscache, A.L. et al. (2011) Effects of BCL-2 over-expression on B cells in transgenic rats and rat hybridomas.
Int Immunol. 23 (10): 625-36. 7. Chylicki, K. et al. (2000) Characterization of the molecular mechanisms for p53-mediated differentiation.
Cell Growth Differ. 11: 561-71. 8. Berrieman, H.K. et al. (2005) The expression of Bcl-2 family proteins differs between nonsmall cell lung carcinoma subtypes.
Cancer. 103: 1415-9. 9. Valgimigli, M. et al. (2003) Serum from patients with acute coronary syndromes displays a proapoptotic effect on human endothelial cells: a possible link to pan-coronary syndromes.
Circulation. 107: 264-70. 10. Shenker, B.J. ,i>et al. (2001) Induction of apoptosis in human T cells by Actinobacillus actinomycetemcomitans cytolethal distending toxin is a consequence of G2 arrest of the cell cycle.
J Immunol. 167: 435-41. 11. Fimognari, C. et al. (2002) Growth inhibition, cell-cycle arrest and apoptosis in human T-cell leukemia by the isothiocyanate sulforaphane.
Carcinogenesis. 23: 581-6.

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