|Application ||FC, IP|
|Calculated MW||37886 Da|
|Immunogen||NK cell clone E57 (Morettaet al.1985).|
|Shelf Life||18 months from date of despatch.|
|Other Names||Killer cell immunoglobulin-like receptor 2DL3, CD158 antigen-like family member B2, KIR-023GB, Killer inhibitory receptor cl 2-3, MHC class I NK cell receptor, NKAT2a, NKAT2b, Natural killer-associated transcript 2, NKAT-2, p58 natural killer cell receptor clone CL-6, p58 NK receptor CL-6, p58.2 MHC class-I-specific NK receptor, CD158b2, KIR2DL3, CD158B2, KIRCL23, NKAT2|
|Target/Specificity||Mouse anti-Human CD158b antibody, clone GL183 recognizes human Killer cell immunoglobulin-like receptor 2DL3, also known as CD158b, KIR-023GB, MHC class I NK cell receptor, p58 natural killer cell receptor clone CL-6 or Natural killer-associated transcript 2. CD158b is a 341 amino acid, ~58 kDa single pass type-1 transmembrane glycoprotein containing two Ig-like C2-type http://www.ebi.ac.uk/interpro/entry/IPR008424 domains. expressed by a subset of NK cells.This antibody also recognises a 50kDa molecule in some NK clones, which is highly homologous to p58.2 in the extracellular domain, but has a shorter cytoplasmic tail (Morettaet al.1985). Both molecules are members of the newly described natural killer cell receptor family.CD158b functions as a receptor specific for HLA Class I molecules, including Cw3 and related HLA-C alleles. Mouse anti-Human CD158b antibody, clone GL183 can restore the lysis by human NK clones of otherwise lysis protected targets expressing Cw3.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human CD158b Antibody, clone GL183 is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Moretta, A. et al. (1990) A novel surface antigen expressed by a subset of human CD3- CD16+ natural killer cells. Role in cell activation and regulation of cytolytic function.J Exp Med. 171 (3): 695-714. 2. Moretta, A. et al. (1993) P58 molecules as putative receptors for major histocompatibility complex (MHC) class I molecules in human natural killer (NK) cells. Anti-p58 antibodies reconstitute lysis of MHC class I-protected cells in NK clones displaying different specificities.J Exp Med. 178 (2): 597-604. 3. Moretta, A. et al. (1995) Existence of both inhibitory (p58) and activatory (p50) receptors for HLA-C molecules in human natural killer cells.J Exp Med. 182 (3): 875-84. 4. Pridgeon, C. et al. (2003) Natural killer cells in the synovial fluid of rheumatoid arthritis patients exhibit a CD56bright,CD94bright,CD158negative phenotype.Rheumatology (Oxford). 42 (7): 870-8. 5. Marget, M. et al. (2005) A HLA-Cw6 specific single-chain antibody fragment (scFv) recognizing a natural killer cell receptor epitope Mol Immunol. 42: 643-9. 6. Poggi, A. et al. (2005) Regulation of gammadelta T cell survival by soluble HLA-I: involvement of CD8 and activating killer Ig-like receptors.Eur J Immunol. 35: 2670-8. 7. Borhis, G. et al. (2013) A peptide antagonist disrupts NK cell inhibitory synapse formation.J Immunol. 190 (6): 2924-30. 8. Poggi, A. et al. (2005) Patients with paroxysmal nocturnal hemoglobinuria have a high frequency of peripheral-blood T cells expressing activating isoforms of inhibiting superfamily receptors.Blood. 106: 2399-408. 9. Valés-Gómez, M. et al. (2003) Expression of the UL16 glycoprotein of Human Cytomegalovirus protects the virus-infected cell from attack by natural killer cells.BMC Immunol. 4:4. 10. Spaggiari, G.M. et al. (2002) Soluble HLA class I molecules induce natural killer cell apoptosis through the engagement of CD8: evidence for a negative regulation exerted by members of the inhibitory receptor superfamily.Blood. 99: 1706-14. 11. Warren, H.S. et al. (2001) Biphasic response of NK cells expressing both activating and inhibitory killer Ig-like receptors.Int Immunol. 13: 1043-52. 12. Ghio, M. et al. (2009) Soluble HLA-I-mediated secretion of TGF-beta1 by human NK cells and consequent down-regulation of anti-tumor cytolytic activity.Eur J Immunol. 39: 3459-68. 13. Spaggiari, G.M. et al. (2003) IFN-gamma production in human NK cells through the engagement of CD8 by soluble or surface HLA class I molecules.Eur J Immunol. 33: 3049-59. 14. Bachelet, I. et al. (2005) The inhibitory receptor IRp60 (CD300a) is expressed and functional on human mast cells.J Immunol. 175: 7989-95. 15. Zimmer, J. et al. (1998) Activity and phenotype of natural killer cells in peptide transporter (TAP)-deficient patients (type I bare lymphocyte syndrome).J Exp Med. 187: 117-22. 16. Castriconi, R,. et al. (2009) NK cells recognize and kill human glioblastoma cells with stem cell-like properties.J Immunol. 182: 3530-9.
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