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>   home   >   Products   >   Primary Antibodies   >   Immunology   >   Anti-Sheep Interleukin-8 Antibody, clone 8M6    

Anti-Sheep Interleukin-8 Antibody, clone 8M6

Mouse Anti-Sheep Monoclonal Antibody

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, E, Func
Primary Accession P36925
Reactivity Sheep
Host Mouse
Clonality Monoclonal
Isotype IgG2a
Clone Names 8M6
Calculated MW 11292 Da
Additional Information
Other Species Dog,Rb,B,Pig,Mustelid,Ferret,Mink,Cat
Immunogen Recombinant ovine IL-8.
Shelf Life 18 months from date of despatch.
Gene ID 443418
Other Names Interleukin-8, IL-8, C-X-C motif chemokine 8, Chemokine (C-X-C motif) ligand 8, CXCL8, IL8
Target/Specificity Mouse anti-Ovine Interleukin-8 antibody, clone 8M6 recognizes ovine interleukin-8 (IL-8), also known as C-X-C motif chemokine 8. IL-8 is a 101amino acid ~16kDa chemoattractant for neutrophils, basophils and T-cells. IL-8 is produced by several cell types including neutrophils, monocytes and macrophages in response to inflammatory stimulation. Mouse anti-ovine Interleukin-8 antibody, clone 8M6 shows no cross-reactivity with ovine IL-1 beta, IL-6, MCP-1 or TNF alpha.Responses to infectious stimuli may vary among ovine species, the response to Mannheimia haemolytica, a causitive agent of pneumonia, peritonitis and gangrenous mastitis in ovids, is exagerrated in Bighorn sheep (Ovis canadensis) compared to domestic sheep (Ovis aries) with significantly elevated IL-8 levels in response to infection (Herndonet al.2010). Mouse anti-ovine interleukin-8 antibody, clone 8M6 has been utilized to identify cells and cell types expressing IL-8 in inflamed porcine tissue (Laursen et al. 2014) showing here also that neutrophils are the predominant cell type expressing IL-8 whilst epithelial and endothelial cells in the vicinity of inflammatory lesions also express the cytokine. Clone 8M6 neutralizes the bioactivity of ovine IL-8. Mouse anti-Ovine Interleukin-8 antibody, clone 8M6 has been used in conjunction with Rabbit anti-Sheep Interleukin-8 antibody (AHP425) for the development of a sensitive ELISA to measuse IL-8 concentrations in feline samples (Croninet al.2014).
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Sheep Interleukin-8 Antibody, clone 8M6 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. Caswell, J.L. et al. (1998) Expression of the neutrophil chemoattractant interleukin-8 in the lesions of bovine pneumonic pasteurellosis.
Vet Pathol. 35: 124-31. 2. Pedersen, L.G. et al. (2002) Identification of monoclonal antibodies that cross-react with cytokines from different animal species.
Vet Immunol Immunopathol. 88 (3-4): 111-22. 3. Aasted, B. et al. (2002) Cytokine profiles in peripheral blood mononuclear cells and lymph node cells from piglets infected in utero with porcine reproductive and respiratory syndrome virus.
Clin Diagn Lab Immunol. 9 (6): 1229-34. 4. Herndon, C.N. et al. (2010) Differential expression of interleukin-8 by polymorphonuclear leukocytes of two closely related species, Ovis canadensisandOvis aries, in response to Mannheimia haemolyticainfection.
Infect Immun. 78: 3578-84. 5. Martel, C.J. & Aasted, B. (2009) Characterization of antibodies against ferret immunoglobulins, cytokines and CD markers.
Vet Immunol Immunopathol. 132:109-15. 6. Zelnickova, P. et al. (2008) Age-dependent changes of proinflammatory cytokine production by porcine peripheral blood phagocytes.
Vet Immunol Immunopathol. 124: 367-78. 7. Bonnefont, C.M. et al. (2012) Genetic susceptibility to S. aureus mastitis in sheep: differential expression of mammary epithelial cells in response to live bacteria or supernatant.
Physiol Genomics. 44: 403-16. 8. Jensen, P.V. et al. (2003) Cytokine profiles in adult mink infected with Aleutian mink disease parvovirus.
J Virol. 77: 7444-51. 9. Singh, B. et al. (2004) Depletion of pulmonary intravascular macrophages inhibits acute lung inflammation.
Am J Physiol Lung Cell Mol Physiol. 286: L363-72. 10. Redondo, E. et al. (2013) Induction of Interleukin-8 and Interleukin-12 in Neonatal Ovine Lung Following Experimental Inoculation of Bovine Respiratory Syncytial Virus.
J Comp Pathol. 150: 434-48. 11. Laursen, H. et al. (2014) Immunohistochemical detection of interleukin-8 in inflamed porcine tissues.
Vet Immunol Immunopathol. 159: 97-102. 12. Boulanger, D. et al. (2003) Increased nuclear factor kappaB activity in milk cells of mastitis-affected cows.
J Dairy Sci. 86: 1259-67. 13. Cronin, J.G. et al. (2015) Enzyme linked immunosorbent assay for quantification of bovine interleukin-8 to study infection and immunity in the female genital tract.
Am J Reprod Immunol. 73 (4): 372-82.

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