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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Anti-Human p53 (aa181-190) Antibody, clone DO-11    

Anti-Human p53 (aa181-190) Antibody, clone DO-11

Mouse Anti-Human Monoclonal Antibody

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P04637
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names DO-11
Calculated MW 43653 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G
Immunogen Recombinant human p53.
Shelf Life 18 months from date of despatch.
Gene ID 7157
Other Names Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53, TP53, P53
Target/Specificity Mouse anti-Human p53 antibody, clone DO-11 recognizes the human Cellular tumor antigen p53, also known as p53 tumour suppressor protein or NY-CO-13. p53 is a 393 amino acid ~53kDa cytoplasmic/ nuclear protein up-regulated in response to DNA damage and is found in a wide variety of transformed cells (UniProt: P04637).DO-11 binds to an epitope within the central region of p53 between amino acids 181 - 190 defining a cryptic epitope exposed in unfolded/ denatured p53 (Shimitzuet al.2006). Nine isoforms of human p53 are produced by alternative splicing and promotor usage, the epitope recognized by clone DO-11 is present in all isoforms. Mouse anti-Human p53 antibody, clone DO-11 recognizes mutant forms of p53 (Warnocket al.2011) and has been used successfully for detection of p53 by western blotting (Weiet al.2012).
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Human p53 (aa181-190) Antibody, clone DO-11 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. Vojtěšek, B. et al. (1995) Conformational changes in p53 analysed using new antibodies to the core DNA binding domain of the protein.
Oncogene. 10: 389-393. 2. Palecek, E. et al. (2001) Binding of p53 and its core domain to supercoiled DNA.
Eur J Biochem. 268: 573-81. 3. Coomber, D.W. and Ward, .R.L. (2001) Isolation of human antibodies against the central DNA binding domain of p53 from an individual with colorectal cancer using antibody phage display.
Clin Cancer Res. 7: 2802-8. 4. Wei, J. et al. (2012) Pathogenic bacterium Helicobacter pylori alters the expression profile of p53 protein isoforms and p53 response to cellular stresses.
Proc Natl Acad Sci U S A. 109: E2543-50. 5. Warnock, L.J. et al. (2011) Aurora A mediates cross-talk between N- and C-terminal post-translational modifications of p53.
Cancer Biol Ther. 12: 1059-68.

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Cat# ABD11615
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