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>   home   >   Products   >   Primary Antibodies   >   Anti-Dog CD11b Antibody, clone CA16.3E10    

Anti-Dog CD11b Antibody, clone CA16.3E10

Mouse Anti-Dog Monoclonal Antibody

  • FC - Anti-Dog CD11b Antibody, clone CA16.3E10  ABD11633
    Published customer image:Optimization of secondary antibody staining concentration for detection of CADO48A. Cells were stained with the primary antibody CADO48A alone followed by (a) 1:10 (b) 1:50 and (c) 1:100 dilutions of a secondary FITC antibody. Optimal detection was seen between 1:50 and 1:100 dilution with CADO48A alone. Secondary FITC concentrations of 1:50 (d) and 1:100 (e) to detect CADO48A on pre-labeled CD11b cells were then evaluated and demonstrated that a 1:50 concentration of FITC was optimal for optimal detection of distinct CADO48A+ cells. Based on these findings, a 1:50 FITC concentration was used in all clinical samples. All cells in these diagrams were gated on P1.From:Sherger et al. BMC Veterinary Research 2012 8:209
  • FC - Anti-Dog CD11b Antibody, clone CA16.3E10  ABD11633
    Published customer image:CD11b+CD14-MHCII- cells suppress T cell proliferation. Facs sorted CD11b+CD14-MHCII- cells isolated from a dog with osteosarcoma or healthy PBMCs were co-incubated with mitogen-stimulated CD4+ and CD8+ T cells isolated from a healthy dog for 72 hs. No stimulated cells were used as negative control. Proliferative responses were measured by 3H-thymidine incorporation from experiments performed in triplicate. CPM, counts per minute. Mean ± SEM are shown.From: Goulart MR, Pluhar GE, Ohlfest JR Identification of Myeloid Derived Suppressor Cells in Dogs with Naturally Occurring Cancer. PLoS ONE 7(3): e33274.
  • FC - Anti-Dog CD11b Antibody, clone CA16.3E10  ABD11633
    Published customer image:Flow cytometer analysis of CD11b integrin expression and the binding of Leishmania promastigotes to canine monocytes. (A) Gate R1 was built separating monocytes from animals naturally infected with L. chagasi by cells size (x axis) and granularity (y axis). (B) Gate with mononuclear cells (C) CD11b positive cells; (D) CD11b expression during the L. chagasi binding to mononuclear cells with the presence of C5D serum. (E) CD11b expression during the L. chagasi binding to mononuclear cells with the absence of C5D serum.From:Sampaio et al. BMC Veterinary Research 2007 3:11
  • FC - Anti-Dog CD11b Antibody, clone CA16.3E10  ABD11633
    Published customer image:Gated myeloid subpopulations evaluated for patient population. All peripheral blood myeloid cells were evaluated using the P1 gate and subsequently gated based on relatively expression levels of CD11b and CADO48A where CADO48Ahi/CD11bhi (gate P2), CADO48Alow/CD11bhi (gate P3) and CADO48Alow/CD11blow (gate P4) likely represent granulocytes/neutrophils, monocytes and MDSCs, respectively.From:Sherger et al. BMC Veterinary Research 2012 8:209
  • FC - Anti-Dog CD11b Antibody, clone CA16.3E10  ABD11633
    Published customer image:CD11b+CD14+MHCII- cells demonstrate ability to suppressive T cell proliferation. (A) CD11b+CD14+MHCII- cells were sorted from peripheral blood sample of an osteosarcoma dog (B) and co-cultured with healthy dog PBMCs in the presence of mitogen for 72 hs. Non-stimulated PBMCs were used as negative control and PBMCs co-cultured with healthy PMNs were used to control for the effect of adding cells to the assay. Proliferative responses were measured by 3H-thymidine incorporation. CPM, counts per minute. The experiment was performed in triplicate. Mean ± SEM are shown.From: Goulart MR, Pluhar GE, Ohlfest JR Identification of Myeloid Derived Suppressor Cells in Dogs with Naturally Occurring Cancer. PLoS ONE 7(3): e33274.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Reactivity Dog
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names CA16.3E10
Additional Information
Other Species Ga,Cat,Mustelid,Pig,B,Mink,Beluga whale
Immunogen Affinity purified beta-2 integrins from splenic lysate
Shelf Life 18 months from date of despatch.
Target/Specificity Mouse anti-Dog CD11b antibody, clone CA16.3E10) is a monoclonal antibody recognising the canine CD11b cell surface antigen, a member of the alpha integrin family. CD11b forms one of the possible alpha chains of the canine leukocyte adhesion complexes (LeuCAMs), these contain a common 95 kDa β chain (CD18) non-covalently bound to either a 150 kDa (CD11c), 165 kDa (CD11b) or 180 kDa (CD11a) α chain (Mooreet al.1990. The CD11/CD18 complex is also known as the CR3 receptor.Canine CD11b is expressed by granulocytes, monocytes, NK cells and some macrophages. Mouse anti-Dog CD11b (CA16.3E10) has been used tio evaluate the effect of anaesthetic administration of CD11b expression on canine neutrophils (Maedaet al.2010) demonstrating attenuation of CD11b expression at high concentrations administered lidocaine hydrochloride and reduced adhesion of neutrophils to endothelium.
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Dog CD11b Antibody, clone CA16.3E10 is for research use only and not for use in diagnostic or therapeutic procedures.
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1. Danilenko, D.M. et al. (1992) Canine leukocyte cell adhesion molecules (LeuCAMs): characterization of the CD11/CD18 family.
Tissue Antigens 40: 13-21. 2. Brodersen, R. et al. (1998) Analysis of the immunological cross reactivities of 213 well charaterized monoclonal antibodies with specificities against various leucocyte surface antigens of human and 11 animal species.
Vet. Immunol. Immunopathol. 64: 1-13. 3. Sampaio, W.M. (2007) In vitro binding and survival assays of Leishmania parasites to peripherical blood monocytes and monocyte-derived macrophages isolated from dogs naturally and experimentally infected with Leishmania chagasi.
BMC Vet Res. 3:11. 4. Maiolini, A. et al. (2012) Toll-like receptors 4 and 9 are responsible for the maintenance of the inflammatory reaction in canine steroid-responsive meningitis-arteritis, a large animal model for neutrophilic meningitis.
J Neuroinflammation. 9: 226. 5. Yuasa, K. et al. (2007) Injection of a recombinant AAV serotype 2 into canine skeletal muscles evokes strong immune responses against transgene products.
Gene Ther. 14: 1249-60. 6. Kamstock, D. et al. (2006) Liposome-DNA complexes infused intravenously inhibit tumor angiogenesis and elicit antitumor activity in dogs with soft tissue sarcoma.
Cancer Gene Ther. 13: 306-17. 7. Sherger, M. et al. (2012) Identification of myeloid derived suppressor cells in the peripheral blood of tumor bearing dogs.
BMC Vet Res. 8: 209. 8. Gregorevic, P. et al. (2009) Evaluation of vascular delivery methodologies to enhance rAAV6-mediated gene transfer to canine striated musculature.
Mol Ther. 17: 1427-33. 9. Figueiredo, M.M. et al. (2013) Expression of Toll-like Receptors 2 and 9 in cells of dog jejunum and colon naturally infected with Leishmania infantum.
BMC Immunol. 14: 22. 10. Thompson, L.A. and Romano, T.A. (2015) Beluga (Delphinapterus leucas) granulocytes and monocytes display variable responses to in vitro pressure exposures.
Front. Physiol. 6:128.

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