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Mouse Anti-Bovine Monoclonal Antibody
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- BACKGROUND
Application 
| WB, FC, E, ELISPOT, Func |
|---|---|
| Primary Accession | P46282 |
| Other Accession | P54349 |
| Reactivity | Bovine |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Clone Names | CC301 |
| Calculated MW | 37050 Da |
| Other Species | H,African Buffalo |
|---|---|
| Purification | Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant |
| Immunogen | Recombinant bovine IL-12. |
| Shelf Life | 18 months from date of despatch. |
| Gene ID | 281857 |
| Other Names | Interleukin-12 subunit beta, IL-12B, Cytotoxic lymphocyte maturation factor 40 kDa subunit, CLMF p40, IL-12 subunit p40, IL12B |
| Target/Specificity | Mouse anti-Bovine Interleukin-12/23 antibody, clone CC301 recognizes the p40 subunit of bovine interleukin-12, and binds to both the free subunit and the intact heterodimer. The p40 subunit is also known as IL-12B and can form a heterodimer with either IL-12A or IL-23A.This antibody may be used as a capture antibody in an ELISA in combination with biotinylated clone CC326 as a detection reagent. |
| Preservative & Stabilisers | Preservative Free |
| Storage | Store at -20℃ only. |
| Precautions | Anti-Bovine Interleukin-12/23 Antibody, clone CC301 is for research use only and not for use in diagnostic or therapeutic procedures. |
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Provided below are standard protocols that you may find useful for product applications.
References
1. Hope, J. C. et al. (2002) Development of detection methods for ruminant interleukin (IL) - 12. J. Immunol. Methods. 266: 117 - 126. 2. Wenz, J.R. et al. (2010) Factors associated with concentrations of select cytokine and acute phase proteins in dairy cows with naturally occurring clinical mastitis.J Dairy Sci. 93: 2458-70. 3. Rinaldi, M. et al (2010) A sentinel function for teat tissues in dairy cows: dominant innate immune response elements define early response to E. colimastitis.Funct Integr Genomics. 10: 21-38. 4. Ferret-Bernard, S. et al. (2011) Mesenteric lymph node cells from neonates present a prominent IL-12 response to CpG oligodeoxynucleotide via an IL-15 feedback loop of amplification.Vet Res. 42:19. 5. Contreras, V. et al. (2010) Existence of CD8a-like dendritic cells with a conserved functional specialization and a common molecular signature in distant mammalian species. J Immunol. 185: 3313-25. 6. Bannerman, D.D. et al. (2004) Escherichia coli and Staphylococcus aureus elicit differential innate immune responses following intramammary infection.Clin Diagn Lab Immunol. 11: 463-72. 7. Davis, T.L. and Pate, J.L. (2007) Bovine luteal cells stimulate proliferation of major histocompatibility nonrestricted gamma delta T cells.Biol Reprod. 77: 914-22. 8. Ferret-Bernard, S. (2010) Cellular and molecular mechanisms underlying the strong neonatal IL-12 response of lamb mesenteric lymph node cells to R-848.PLoS One. 5: e13705. 9. Shoda, L.K. et al. (2001) DNA from protozoan parasites Babesia bovis, Trypanosoma cruzi, and T. brucei is mitogenic for B lymphocytes and stimulates macrophage expression of interleukin-12, tumor necrosis factor alpha, and nitric oxide.Infect Immun. 69: 2162-71. 10. Souza, M. et al. (2008) Pathogenesis and immune responses in gnotobiotic calves after infection with the genogroup II.4-HS66 strain of human norovirus.J Virol. 82: 1777-86. 11. Verhelst, D. et al. (2014) Parasite distribution and associated immune response during the acute phase of Toxoplasma gondii infection in sheep.BMC Vet Res. 10: 293. 12. Beechler BR et al. (2015) Enemies and turncoats: bovine tuberculosis exposes pathogenic potential of Rift Valley fever virus in a common host, African buffalo (Syncerus caffer).Proc Biol Sci. 282 (1805): . 13. Pomeroy B et al. (2015) Monocyte-derived dendritic cells from late gestation cows have an impaired ability to mature in response to E. coli stimulation in a receptor and cytokine-mediated fashion.Vet Immunol Immunopathol. 167 (1-2): 22-9.
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