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CD203a Antibody, clone PM18-7 (Monoclonal Antibody) (FITC)

Mouse Anti-Pig Monoclonal Antibody

  • FC - CD203a Antibody, clone PM18-7 (Monoclonal Antibody) (FITC) ABD11667
    Published customer image:Overview of flow cytometry analyses. (A) Gating strategy for identification of monocytes and macrophages. The peripheral blood is shown. Designation of population shown within each dot-plot is indicated above the dot-plot. Leukocytes were identified as viable (a) non-doublet (b) cells with typical light scatter properties of leukocytes (c). Then, macrophages were gated simply as CD203ahi leukocytes (d) and marked with blue color. Monocytes were gated as CD203alow/- SWC8-(e) CD172ahi(f) leukocytes where the CD203alow/- region was defined as the complementary region to the CD203ahi region. Then, SLA-DR+ monocytes were marked with red color and SLA-DR- monocytes were marked with green color (g). SLA-DR- region was defined as the complementary region to the SLA-DR+ region. Gating order is shown in the scheme (h). (B) Representative CD163 vs. CD14 dot-plots of macrophages (blue) and monocyte subpopulations (green: SLA-DR–, red: SLA-DR+) in various body compartments of control and APP-infected pigs.From: Ondrackova P, Leva L, Kucerova Z, Vicenova M, Mensikova M, Faldyna M. Distribution of porcine monocytes in different lymphoid tissues and the lungs during experimental Actinobacillus pleuropneumoniae infection and the role of chemokines. Vet Res. 2013 Oct 17;44:98.
  • FC - CD203a Antibody, clone PM18-7 (Monoclonal Antibody) (FITC) ABD11667
    Published customer image:Single colour flow cytometry analysis of changes in several immunologically relevant cell surface molecules of bone marrow-derived dendritic cells after 48 h of incubation with different PRRSV strains at a multiplicity of infection of 0.01 (as determined by titration in porcine alveolar macrophages). Grey histograms show cells stained with an irrelevant isotype-matched antibody and a secondary FITC or phycoerithrin conjugated antibody. Blue histograms show uninfected cells and red line histograms correspond to PRRSV-infected cells. Averages and variation coefficients are included. Grey values show the uninfected cell parameters and black values correspond to PRRSV-infected cells. A) SLA-I, SLA-II, CD80/86, CD14; B) SwC3, SwC9, CD163, CD16.From: Gimeno M, Darwich L, Diaz I, de la Torre E, Pujols J, Martín M, Inumaru S, Cano E, Domingo M, Montoya M, Mateu E. Cytokine profiles and phenotype regulation of antigen presenting cells by genotype-I porcine reproductive and respiratory syndrome virus isolates. Vet Res. 2011 Jan 18;42:9.
  • FC - CD203a Antibody, clone PM18-7 (Monoclonal Antibody) (FITC) ABD11667
    Staining of pig alveolar macrophages with Mouse anti Pig CD203a followed by Goat anti Mouse IgG:FITC
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Reactivity Pig
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names PM18-7
Additional Information
Shelf Life 18 months from the date of despatch.
Target/Specificity Mouse anti-Pig CD203a, clone PM18-7 recognizes porcine CD203a, originally clustered as SWC9 at the Second International Swine CD Workshop (Dominguezet al.1998) and later identified as the porcine homologue of human ecto-nucleotidepyrophosphatase / phosphodiesterase 1 or NPP1(Petersenet al.2007).Mouse anti-Pig CD203a was originally reported to immunoprecipitate two bands, one of >205 kDa and one of ~130 kDa (Dominguezet al.1998) under both reducing and non-reducing conditions. Subsequent studies suggest that CD203a migrates as a homodimer of ~260 kDa under non-reducing conditions and a 130 kDa monomer under reducing conditions (Petersenet al.2007) from preparations of porcine alveolar macrophages.CD203a is expressed widely in macrophage populations with notably high levels on alveolar macrophages (Petersenet al.2007), it is not expressed on monocyte populations (McCulloughet al.1997).SWC1a, expressed at very much higher levels on monocytes than mature macrophages and CD203a (SWC9), expressed exclusively on mature tissue macrophages have been used as markers of monocyte-macrophage differentiation (Sanchezet al.1999).
Preservative & Stabilisers 0.09% Sodium Azide (NaN3); 1% Bovine Serum Albumin
Storage Store at +4℃ or -20℃.
PrecautionsCD203a Antibody, clone PM18-7 (Monoclonal Antibody) (FITC) is for research use only and not for use in diagnostic or therapeutic procedures.
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1. McCullough, K. C. et al (1997) Phenotype of porcine monocytic cells: modulation of surface molecule expression upon monocyte differentiation into macrophages.
Vet Immunol Immunopathol 58: 265 - 75. 2. McCullough, K. C. et al (1999) Intermediate stages in monocyte-macrophage differentiation modulate phenotype and susceptibility to virus infection.
Immunology 98: 203 - 12. 3. Boersma, W. et al. (2001) Summary of workshop findings for porcine B cell markers.
Vet Immunol Immunopathol. 80: 63 - 78. 4. Dominguez, J. et al. (1998) Porcine myelomonocytic markers: summary of the second international swine CD workshop.
Vet Immunol Immunopathol. 60: 329 - 341. 5. Dominguez, J. et al. (1998) Workshop studies with monoclonal antibodies identifying a novel porcine differentation antigen SWC9.
Vet Immunol Immunopathol. 60: 343 - 349. 6. Petersen, C.B. et al. (2007) Porcine ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (NPP1/CD203a):cloning, transcription, expression, mapping, and identification of anNPP1/CD203a epitope for swine workshop cluster 9 (SWC9) monoclonal antibodies.
Dev Comp Immunol. 31: 618 - 631. 7. Basta, S. et al. (1999) Modulation of monocytic cell activity and virus susceptibility during differentiation into macrophages.
J Immunol. 162: 3961 - 9. 8. Gimeno, M. et al. (2011) Cytokine profiles and phenotype regulation of antigen presenting cells by genotype-I porcine reproductive and respiratory syndrome virus isolates.
Vet Res. 42: 9. 9. Sanchez, C. et al. (1999) The porcine 2A10 antigen is homologous to human CD163 and related to macrophage differentiation.
J Immunol. 162: 5230 - 7. 10. Cantu, E. et al. (2006) Depletion of pulmonary intravascular macrophages prevents hyperacute pulmonary xenograft dysfunction.
Transplantation 81: 1157 - 64. 11. Basta, S. et al. (2001) Lipopolysaccharide and phorbol 12-myristate 13-acetate both impair monocyte differentiation, relating cellular function to virus susceptibility.
Immunology 103: 488 - 97. 13. Lithgow, P. et al. (2014) Correlation of cell surface marker expression with African swine fever virus infection.
Vet Microbiol. 168: 413 - 9. 14. Ondrackova, P. et al. (2013) Phenotypic characterisation of the monocyte subpopulations in healthy adult pigs and< i>Salmonella-infected piglets by seven-colour flow cytometry.
Res Vet Sci. 94: 240 - 5. 12. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.

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