|Application ||IHC-F, FC, IP|
|Calculated MW||51640 Da|
|Shelf Life||18 months from date of despatch.|
|Other Names||T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, CD4, CD4|
|Target/Specificity||Mouse anti-Dog CD4 antibody (CA13.1E4), a monoclonal antibody specific for canine CD4. CA13.1E4 was clustered at the first Canine Leukocyte Antigen Workshop (Claw) [Cobboldet al.1992 ] and identifies, by immunoprecipitation a 60kDa monomeric protein under both reducing and non-reducing conditions. Canine CD4 is a surface glycoprotein expressed by non CD8 expressing T lymphocytes, with similar developmental and functional characteristics to T helper cells described in other mammalian species. Mouse anti-Dog CD4 also recognizes a population of CD8 positive cells in the canine thymus (Mooreet al.1992).CD4 expression has also been reported on a population of CD3 positive peripheral blood T lymphocytes which are double positive for CD4 and CD8 (Bismarcket al.2012 ). Similar reports have been made for cynomolgus monkey (Nam et al.2000), pig (Saalmulleret al.1987,Pescowitzet al.1990) as well as rat, chicken and human (reviewed Zuckermann 1999).Uniquely amongst mammalian species clone CA13.1E4 also recognizes CD4 expressed on canine neutrophils at a similar density to that expressed on canine T helper cells. The functional significance of this is not understood and remains enigmatic in view of the roles described for CD4 in canine and other mammalian species (Mooreet al.1992).Clone CA13.1E4 also demonstrates immunohistological staining of splenic marginal zone macrophages (Mooreet al.1992). Other macrophage populations, such as splenic red pulp macrophages and Langerhans cells do not stain with clone CA13.1E4.Canine CD4 positive T cells can be further characterized according to their simultaneous expression of CD45RA as recognized by Rat anti-Canine anti-CD45RA clone CA4.1D3 in a manner analogous to that seen with human T cells (Mooreet al.1992).Clone CA13.1E4 has been used amongst a large panel of anti-canine monoclonal antibodies for the study of various lymphoploriferative diseases. T lymphocytes in large granular lymophocyte (LGL) lymphocytosis are negative for CD4 (McDonough and Moore 2000 ). Dogs with chronic myelogenous leukemia demonstrate CD4 positive staining on neutrophils (Tarrantet al.2001). CD4 expression varies considerably between various canine leukemias (Villiers 2002).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Dog CD4 Antibody, clone CA13.1E4 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Moore, P.F. et al. (1992) Monoclonal antibodies specific for canine CD4 and CD8 define functional T-lymphocyte subsets and high-density expression of CD4 by canine neutrophils.Tissue Antigens 40(2): 75-85. 2. Cobbold, S. & Metcalfe, S. (1994) Monoclonal antibodies that define canine homologues of human CD antigens: Summary of the first International Canine Leucocyte Antigen Workshop (CLAW).Tissue Antigens 43(3): 137-154. 3. Veenhof, E.Z. et al. (2011) Characterisation of T cell phenotypes, cytokines and transcription factors in the skin of dogs with cutaneous adverse food reactions.Vet J. 187 (3): 320-4.
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