|Application ||IHC-F, FC, IP|
|Purification||Purified IgG prepared from ascites|
|Immunogen||Chicken thymocytes and Ig -ve peripheral blood mononuclear cells.|
|Shelf Life||18 months from date of despatch.|
|Other Names||T-cell surface glycoprotein CD3 epsilon chain, CD3e, CD3E|
|Target/Specificity||Mouse anti-Chicken CD3, clone CT-3 recognizes the chicken CD3 cell surface antigen, a complex of at least three polypeptides of 20, 19 and 17kDa expressed by T cells (Chenet al.1986). The antibody also co-precipitates a 90kDa heterodimer from digitonin solubilized T cell lysates, which can be reduced to two polypeptides of 40kDa and 50 kD.In the chicken, CD3 is expressed by approximately 30% of thymocytes, 70% of peripheral blood mononuclear cells, but|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Chicken CD3 Antibody, clone CT-3 is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Chen, C. L. et al. (1986) Identification of a T3/T cell receptor complex in chickens.J. Exp. Med. 164: 375-380. 2. Mukamoto, M. & Kodama, H. (2000) Regulation of early chicken thymocyte proliferation by transforming growth factor-beta from thymic stromal cells and thymocytesVet Immunol Immunopathol. 77: 121 - 132 3. Chaussé, A.M. et al. (2011) Expression of toll-like receptor 4 and downstream effectors in selected cecal cell subpopulations of chicks resistant or susceptible to salmonella carrier state.Infect Immun. 79: 3445-54. 4. Balic, A. et al. (2014) Visualisation of chicken macrophages using transgenic reporter genes: insights into the development of the avian macrophage lineage.Development. 141: 3255-65.
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