|Application ||WB, IHC-P, IHC-F, IF, FC, E|
|Shelf Life||18 months from date of despatch.|
|Target/Specificity||Mouse anti-5-Methylcytidine antibody, clone 33D3 recognizes 5-MethylCytidine, a modified base found in the DNA of plants and vertebrates.Methylation of DNA is an epigenetic process that stably alters the expression of genes in cells as they divide and differentiate into specific tissues. The resulting change is normally permanent and unidirectional, preventing a cell from reverting to a stem cell or converting into another type of tissue. In cancer biology, DNA hypermethylation is associated with gene silencing while hypomethylation is reported to be associated with disease progression (Sincic & Herceg, 2011).Mouse anti-5-MeCyd antibody, clone 33D3 is specific to the methylated base and has minimal reactivity to non-methylated cytidine or cytosine (Reynaudet al.1992)Mouse anti-5-MeCyd antibody, clone 33D3 has been reported for use in Methylated DNA immunoprecipitation (MeDIP) (Ponteset al.2007).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-5-Methylcytidine Antibody, clone 33D3 is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Reynaud, C. et al. (1992) Monitoring of urinary excretion of modified nucleosides in cancer patients using a set of six monoclonal antibodies. Cancer Lett. 61: 255-262. 2. Habib, M. et al. (1999) DNA Global Hypomethylation in EBV - Transformed Interphase Nuclei. Exp. Cell. Res. 249: 46-53. 3. Hernandez-Blazquez, F. et al. (2000) Evaluation of global DNA hypomethylation in human colon cancer tissues by immunohistochemistry and image analysis. Gut. 47: 689-693. 4. Giraldo, A. M. et al. (2007) DNA methylation and histone acetylation patterns in cultured bovine fibroblastsfor nuclear transfer.Mol. Reprod. Dev. 74: 1514-1524. 5. Shen, R. et al. (2009) Reversibility of aberrant global DNA and estrogen receptor-alpha gene methylation distinguishes colorectal precancer from cancer.Int J Clin Exp Pathol. 2:21-33. 6. Pontes, O. et al. (2007) Postembryonic establishment of megabase-scale gene silencing in nucleolar dominance.PLoS One. 2: e1157. 7. Yang, F. et al. (2010) Trichostatin A and 5-azacytidine both cause an increase in global histone H4 acetylation and a decrease in global DNA and H3K9 methylation during mitosis in maize.BMC Plant Biol. 10: 178. 8. Suter, J.D. et al. (2010) Label-free DNA methylation analysis using opto-fluidic ring resonators.Biosens Bioelectron. 26: 1016-20. 9. Li, Y. and O'Neill, C. (2012) Persistence of cytosine methylation of DNA following fertilisation in the mouse.PLoS One. 7:e30687. 10. Çelik, S. et al. (2014) The Exit of Mouse Embryonic Fibroblasts from the Cell-Cycle Changes the Nature of Solvent Exposure of the 5'-Methylcytosine Epitope within Chromatin.PLoS One. 9: e92523. 11. Li, Y. & O'Neill, C. (2013) 5'-Methylcytosine and 5'-hydroxymethylcytosine each provide epigenetic information to the mouse zygote.PLoS One. 8 (5): e63689. 12. Leter, G. et al. (2014) Exposure to perfluoroalkyl substances and sperm DNA global methylation in Arctic and European populations.Environ Mol Mutagen. 55 (7): 591-600. 13. Desjobert, C. et al. (2015) Combined analysis of DNA methylation and cell cycle in cancer cells.Epigenetics. 10 (1): 82-91.1. Sinčić, N & Herceg, Z. (2011) DNA methylation and cancer: ghosts and angels above the genes.Curr Opin Oncol. 23: 69-76.
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