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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   Anti-Human p21/WAF1 Antibody, clone WA-1    

Anti-Human p21/WAF1 Antibody, clone WA-1

Mouse Anti-Human Monoclonal Antibody

  • WB - Anti-Human p21/WAF1 Antibody, clone WA-1  ABD11758
    p21/WAF1 transfected cell lysate probed with Mouse anti Human p21/WAF1
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P38936
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names WA-1
Calculated MW 18119 Da
Additional Information
Purification Ig fraction prepared by ammonium sulphate precipitation
Immunogen Human p21 protein
Shelf Life 18 months from date of despatch.
Gene ID 1026
Other Names Cyclin-dependent kinase inhibitor 1, CDK-interacting protein 1, Melanoma differentiation-associated protein 6, MDA-6, p21, CDKN1A, CAP20, CDKN1, CIP1, MDA6, PIC1, SDI1, WAF1
Target/Specificity ABD11758 recognises the human cyclin-dependent kinase inhibitor p21/WAF1, which is an important cell cycle regulatory protein, also known as CIP1.The p21 protein inhibits cell cycle progression at both the G1 and G2 checkpoints. The expression of p21 is inducible by a wide range of stress stimuli.ABD11758 detects a band of approximately 21kD in MCF-7 cell lysates following UV radiation.
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Human p21/WAF1 Antibody, clone WA-1 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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1. Horky, M. et al. (1997) nduction of cell-cycle inhibitor p21 in rat ventricular myocytes during early postnatal transition from hyperplasia to hypertrophy.
Physiol. Res. 46: 233-235. 2. Kotala, V. et al. (2001) Potent induction of wild-type p53-dependent transcription in tumour cells by a synthetic inhibitor of cyclin-dependent kinases.
Cell. Mol. Life Sci. 58: 1333-1339. 3. Kolar, Z. et al. (2000) Relation of Bcl-2 expression to androgen receptor, p21WAF1/CIP1, and cyclin D1 status in prostate cancer.
Mol. Pathol. 53: 15-18. 4. Khanna, S.J. et al. (2001) V-Abl protein-tyrosine kinase up-regulates p-21 WAF-1 in cell cycle arrested and proliferating myeloid cells.
J. Biol. Chem. 276: 11143-11150. 5. Scott, M.T. et al. (2002) PDK1-dependent activation of atypical PKC leads to degradation of the p21 tumour modifier protein.
EMBO J. 21: 6771-6780. 6. Blaydes, J.P. et al. (2001) Stoichiometric phosphorylation of human p53 at Ser315 stimulates p53-dependent transcription.
J Biol Chem. 276: 4699-708.

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Cat# ABD11758
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