|Application ||WB, E|
|Calculated MW||24770 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant|
|Immunogen||Full - length SOCS3 protein.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Suppressor of cytokine signaling 3, SOCS-3, Cytokine-inducible SH2 protein 3, CIS-3, STAT-induced STAT inhibitor 3, SSI-3, SOCS3, CIS3, SSI3|
|Target/Specificity||Mouse anti-Human SOCS-3 antibody, clone SO1 recognizes the human suppressor of cytokine signaling 3 protein, also known as SOCS-3, cytokine-inducible SH2 protein 3 or STAT-induced STAT inhibitor 3. SOCS-3 is a 225 amino acid, 30kDa member of the suppressor of cytokine signaling family (Starret al.1997) containing a single SH2 and a single SOCS box domain. SOCS-3 acts as a negative regulator of Janus Kinase (JAK) and signal transducer and activators of transcription (STAT) signaling pathways.SOCS-3 specifically acts as a negative feedback regulator for the STAT3 pathways, by directly binding to the Tyr-759 motif of the IL-6 signal-transducing receptor subunit gp130, thereby preventing the IL-6 activation of STAT3. The expression of SOCS-3 is inducible by numerous factors, including IL-6, IL-10, Toll-like receptor (TLR) agonists and IFN gamma, making this protein the focus of research into inflammatory diseases, such as Arthritis, Colitis, and Crohn’s Disease (Elliott and Johnstone 2004). SOCS-3 expression has also been shown to affect IFN alpha sensitivity in tumour studies (Brender et al.2001).Notch signaling plays a critical role in the determination of M1 versus M2 macrophage polarization, and that RBPJ–mediated Notch signaling, regulates this polarization through SOCS-3 (Narayana and Balaji 2008,Schroeder and Just 2000).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human SOCS-3 Antibody, clone SO1 is for research use only and not for use in diagnostic or therapeutic procedures.|
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5. De Rosa, V. et al. (2007) A key role of leptin in the control of regulatory T cell proliferation.Immunity. 26: 241-55.1. Lehmann, U. et al. (2003) SHP2 and SOCS3 contribute to Tyr-759-dependent attenuation of interleukin-6 signaling through gp130.J Biol. Chem. 278(1): 661-671. 2. Suzuki, A. et al. (2001) CIS3/SOCS3/SSI3 plays a negative regulatory role in STAT3 activation and intestinal inflammation.J. Exp. Med. 193(4): 471-481. 3. Lang, R. et al. (2003) SOCS3 regulates the plasticity of gp130 signaling.Nature Immunology 4(6): 546-550. 4. Sakai, I. et al. (2002) Constitutive expression of SOCS3 confers resistance to IFN-alpha in chronic myelogenous leukemia cells. Blood 100(8): 2926-2930.
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