|Application ||WB, IP|
|Calculated MW||72066 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant|
|Immunogen||Recombinant fragment corresponding to amino acids 5 - 360 of human syk.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Tyrosine-protein kinase SYK, 22.214.171.124, Spleen tyrosine kinase, p72-Syk, SYK|
|Target/Specificity||ABD11776 recognizes protein tyrosine kinase p72syk (syk), a 72kDa non-receptor tyrosine kinase, critical for immunoreceptor signaling in haematopoietic cells, but also expressed by nonhaematopoietic cells, including fibroblasts, endothelial cells and hepatocytes.Syk plays an important role in mast cell response, initiated by the aggregation of the IgE high affinity receptor Fc epsilon R1. Phosphorylation of ITAMs within Fc receptor R1 by another tyrosine kinase lyn, results in the recruitment of syk, which is then responsible for the further phosphorylation of numerous target proteins within many different signal transduction pathways.Further studies have shown that syk also plays a major role in B cell receptor-mediated apoptosis, proliferation and haematopoiesis. Syk is also reported to associate with protein kinase C alpha during platelet activation.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Syk Antibody, clone SYK-01 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Amoui, M. et al. (1997) Direct interaction of Syk and Lyn protein tyrosine kinases in rat basophilic leukemia cells activated via type I Fc epsilon receptors.Eur. J. Immunol. 27(1): 321-328. 2. Tolar, P. et al. (1997) Protein tyrosine kinase Syk is involved in Thy-1 signaling in rat basophilic leukemia cells.Eur. J. Immunol. 27(12): 3389-3397. 3. Manetz, T.S. et al. (2001) Vav1 regulates phospholipase C gamma activation and calcium responses in mast cells.Mol. Cell. Biol. 21(11): 3763-3774. 4. Swann, P.G.et al. (1999) Requirement for a negative charge at Threonine 60 of the FcR gamma for complete activation of Syk.J. Biol. Chem. 274(33): 23068-23077. 5. Arudchandran, R.et al. (2000) The src homology 2 domain of Vav is required for its compartmentation to the plasma membrane and activation of c-Jun NH2-terminal kinase 1.J. Exp. Med. 191(1): 47-59.
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