|Application ||WB, IHC-F, FC|
|Calculated MW||270835 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||Synthetic peptide corresponding to cdc10-NCR region within mouse Notch1.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Neurogenic locus notch homolog protein 1, Notch 1, Motch A, mT14, p300, Notch 1 extracellular truncation, NEXT, Notch 1 intracellular domain, NICD, Notch1, Motch|
|Target/Specificity||Mouse anti-Mouse Notch 1 antibody, clone mN1A specifically recognizes Notch 1, one of the four major transmembrane receptors (Notch 1-4) of the Notch signalling pathway, which is activated through binding to DSL domain-containing membrane-bound specific ligands. The Notch signalling pathway is an evolutionarily conserved pathway in multi-cellular organisms, which is vital for cell-cell communication, important during fundamental developmental and physiological processes, including regulation of cell fate decisions during neuronal, cardiac and endocrine development, stem cell haematopoiesis, thymic T-cell development, and both tumour progression and suppression. Ligation of Notch receptors by their specific ligands, Jagged1 (CD339), Jagged2, Delta like-1 (DLL1), DLL3 and DLL4, on physically adjacent signal receiving cells, induces proteolysis of the receptors by ADAM-family metalloproteases and gamma-secretase complex, within the transmembrane domain, releasing the Notch intracellular domain (NICD) to translocate to the nucleus. Subsequent signal transduction then occurs through either the CSL-NICD-Mastermind complex cascade (canonical pathway), or NF-kappaB-NICD and CSL-NICD-Deltex complex signalling cascades (non-canonical pathway). The canonical pathway inhibits the differentiation of stem cells or progenitor cells, whilst the non-canonical pathway promotes differentiation.Notch 1 is expressed in a range of cells including haematopoietic cells in mouse foetal liver, adult thymus and bone marrow. Notch 1 signalling plays a role in follicular differentiation, tissue homeostasis, and in both CD4+ and CD8+ cell maturation in the thymus. Studies have also implicated Notch 1 in the regulation of lymphopoeisis, myelopoiesis, and neurogenesis.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Mouse Notch 1 Antibody, clone mN1A is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Ray, W.J. et al. (1999) Evidence for a physical interaction between presenilin and Notch.Proc. Natl. Acad. Sci. USA. 96:3263-3268. 2. Huppert, S.S. et al. (2000) Embryonic lethality in mice homozygous for a processing-deficient allele of Notch1.Nature 405: 966-970. 3. Espinosa, L. et al. (2002) p65-NFkappa B synergizes with Notch to activate transcription by triggering cytoplasmic translocation of the nuclear receptor corepressor N-CoR.J. Cell. Sci. 115: 1295-1303. 5. Ren, M. and Cowell, J.K. (2011) Constitutive Notch pathway activation in murine ZMYM2-FGFR1-induced T-cell lymphomas associated with atypical myeloproliferative disease.Blood. 117: 6837-47.4. Kang-Decker, N. et al. (2004) Loss of CBP causes T cell lymphomagenesis in synergy with p27Kip1 insufficiency.Cancer Cell. 5: 177-189.
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