|Application ||FC, IP|
|Clone Names||Du CD8-1|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||293T cells expressing Pekin Duck CD8 alpha.|
|Shelf Life||18 months from date of despatch.|
|Target/Specificity||Mouse anti-Duck CD8 alpha antibody, clone Du CD8-1 recognizes Pekin duck CD8 alpha (CD8a). CD8a is shown to be expressed by thymocytes, splenocytes, peripheral lymphoid cells and the vast majority of bursal cells.Since the majority of avian immune studies have been carried out on chickens, relatively little is known about the immune system of ducks, though there is a resemblance between the main lymphoid organs: the spleen, thymus and bursa of Fabricius. At the cellular level, studies have shown that like mammalian T cells, duck lymphocytes are responsive to phytohaemagglutinin (PHA).Double-staining with clone Du CD8-1 and MCA2480 Mouse anti-Duck IgY light chain clone 14A3 revealed the presence of a CD8high/14A3- cytotoxic T cell population and a CD8low/14A3+ B cell population in duck spleen and also revealed a high percentage of CD8+/14A3+ cells in duck bursa (Kothlowet al.1985). Mouse anti-Duck CD8 alpha antibody, clone Du CD8-1 has been shown to not react with Mallard.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Duck CD8 alpha Antibody, clone Du CD8-1 is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Kothlow, S. et al. (2005) Characterization of duck leucocytes by monoclonal antibodies.Dev Comp Immunol. 29 (8): 733-48. 2. Yu, X. et al. (2012) Attenuated Salmonella typhimurium delivering DNA vaccine encoding duck enteritis virus UL24 induced systemic and mucosal immune responses and conferred good protection against challenge.Vet Res. 43: 56. 3. Lian, B. et al. (2011) Induction of immune responses in ducks with a DNA vaccine encoding duck plague virus glycoprotein C.Virol J. 8: 214. 5. Chen, S. et al. (2015) Immunobiological activity and antiviral regulation efforts of Chinese goose (Anser cygnoides) CD8α during NGVEV and GPV infection.Poult Sci. 94 (1): 17-24.1. Higgins, D.A. & Teoh, C.S. (1988) Duck lymphocytes. II. Culture conditions for optimum transformation response to phytohaemagglutinin.J Immunol Methods. 106 (1): 135-45.
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