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Anti-Pig CD117 Antibody, clone 2B8/BM

Mouse Anti-Pig Monoclonal Antibody

  • FC - Anti-Pig CD117 Antibody, clone 2B8/BM  ABD11826
    Porcine Lymphocytes stained with Mouse anti Porcine CD117: Alexa Fluor® 488
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q2HWD6
Reactivity Pig
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names 2B8/BM
Calculated MW 109135 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen Porcine bone marrow cells (BMC).
Shelf Life 18 months from date of despatch.
Gene ID 396810
Other Names Mast/stem cell growth factor receptor Kit, SCFR,, Proto-oncogene c-Kit, Tyrosine-protein kinase Kit, CD117, KIT
Target/Specificity Mouse anti-Pig CD117, clone 2B8/BM is specific for porcine CD117, also known as c-kit, a 155 kDa type I transmembrane protein with protein tyrosine kinase activity, which plays an important role in early hematopoiesis (Perezet al.2007). Hematopoietic stem cells (HSC) of bone marrow have multilineage differentiation potential and an extensive capacity for self-renewal. The majority of adult bone marrow hematopoietic progenitor cells are CD117+ and have been used successfully in xenograft transplantation models for long term survival of grafts, without symptoms of graft-versus-host disease (GVHD). Mouse anti-pig CD117, clone 2B8/BM recognizes CD117 on a small subset of porcine bone marrow progenitor cells and therefore provides an alternative tool from the previously used c-kit ligand stem cell factor, for the isolation and enrichment of porcine stem cells.
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Pig CD117 Antibody, clone 2B8/BM is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. Pérez C et al. (2007) Characterisation of porcine bone marrow progenitor cells identified by the anti-c-kit (CD117) monoclonal antibody 2B8/BM.
J Immunol Methods. 321 (1-2): 70-9. 2. Hatzistergos, K.E. et al. (2010) Bone marrow mesenchymal stem cells stimulate cardiac stem cell proliferation and differentiation.
Circ Res. 107: 913-22. 3. Escalona, Z. et al. (2014) Molecular characterization and expression of porcine Siglec-5.
Dev Comp Immunol. 44 (1): 206-16.1. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.

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Cat# ABD11826
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