|Application ||WB, E|
|Calculated MW||187148 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein A|
|Immunogen||Native purified human C3a|
|Shelf Life||18 months from date of despatch.|
|Other Names||Complement C3, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, Complement C3 beta chain, C3-beta-c, C3bc, Complement C3 alpha chain, C3a anaphylatoxin, Acylation stimulating protein, ASP, C3adesArg, Complement C3b alpha' chain, Complement C3c alpha' chain fragment 1, Complement C3dg fragment, Complement C3g fragment, Complement C3d fragment, Complement C3f fragment, Complement C3c alpha' chain fragment 2, C3, CPAMD1|
|Target/Specificity||ABD11827 recognises 9 kDa complement component 3a (C3a) which is present in the blood serum. C3a is generated over the course of complement activation where complement 3 (C3) convertases cleave C3 to C3a and C3b, which is further degraded into iC3b and C3dg/C3d. C3 is crucial in the induction of tolerance generated when an antigen is introduced into immunoprivileged sites and this is exploited by pathogens and cancer cells to evade the immune system by inhibiting complement activation. C3a mediates local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. C3a is expressed at significantly higher levels in colorectal cancer.ABD11827 shows minimal reactivity with other complement proteins in radioimmunoassays and haemagglutination. Clone 10-21 has been reported to work in western blotting applications.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human C3a Antibody, clone 10-21 is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Hugli, T. (1975) Human anaphylatoxin (C3a) from the third component of complement. Primary structure. J. Biol. Chem. 250:8293-8301. 2. Habermann, J. et al. (2006) Increased serum levels of complement C3a anaphylatoxin indicate the presence of colorectal tumors. Gastroenterology. 131:1020-1029.
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