|Application ||WB, IF, IP|
|Calculated MW||122375 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant|
|Immunogen||Recombinant protein corresponding to amino acids 281-419 of human Bub1 protein.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Mitotic checkpoint serine/threonine-protein kinase BUB1, hBUB1, 22.214.171.124, BUB1A, BUB1, BUB1L|
|Target/Specificity||ABD11865 detects Bub1, a protein kinase involved in spindle checkpoint function during mitosis. Bub1 co-operates with Aurora B kinase to inhibit Cdc20-mediated activation of the anaphase promoting complex/cyclosome (APC/C) until all the chromosomes correctly align on the microtubule spindle apparatus via their kinetochores, thus ensuring high fidelity mitotic chromosome segregation. Bub1 is also required for assembly of a functional inner centromere, sister chromatid cohesion via targeting of the Shugoshin protein, and metaphase congression. Mutation or aberrant Bub1 expression is associated with chromosomal instability, aneuploidy, and human cancer.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human BUB1 Antibody, clone 14H5 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Morrow, C.J. et al. (2005) Bub1 and aurora B cooperate to maintain BubR1-mediated inhibition of APC/CCdc20. J. Cell Sci. 118: 3639-3652. 2. Meraldi, P. and Sorger, P.K. (2005) A dual role for Bub1 in the spindle checkpoint and chromosome congression. EMBO J. 24: 1621-1633. 3. Vanoosthuyse, V. and Hardwick, K.G. (2005) Bub1 and the multilayered inhibition of Cdc20-APC/C in mitosis. Trends Cell Biol. 15: 231-233.
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