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>   home   >   Products   >   Primary Antibodies   >   Microbiology   >   Anti-Epstein-Barr Virus Nuclear Antigen Antibody, clone E1-2.5    

Anti-Epstein-Barr Virus Nuclear Antigen Antibody, clone E1-2.5

Mouse Anti-Viral Monoclonal Antibody

  • FC - Anti-Epstein-Barr Virus Nuclear Antigen Antibody, clone E1-2.5  ABD11874
    Published customer image:Mouse anti Epstein-Barr Virus Nuclear Antigen antibody, clone E1-2.5 used for the detection of EBNA-1 in cell extracts by western blotting.Image caption:(a) Epitope comparison amongst the variants and those observed in the 3 cell lines studied. Red letters denote amino acid mutation. (b) Quantitation of HLA-A0201/LMP1,/LMP2A and/EBNA1 on CCRF-SB, RPMI-6666 and C666-1A2 using TCR-like mAbs based on flow cytometry. Error bars are standard deviation from an average of three independent experiments. **p < 0.01. n.d. denotes not detectable. (c) LMP1, LMP2A and EBNA1 in each of the cell lines was determined using RT-PCR and Western blot. &beta;-actin was used as control.From: Sim AC, Too CT, Oo MZ, Lai J, Eio MY, Song Z, Srinivasan N, Tan DA, Pang SW, Gan SU, Lee KO, Loh TK, Chen J, Chan SH, MacAry PA. Defining the expression hierarchy of latent T-cell epitopes in Epstein-Barr virus infection with TCR-like antibodies.Sci Rep. 2013 Nov 18;3:3232.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P03211
Reactivity Virus
Host Mouse
Clonality Monoclonal
Isotype IgG2b
Clone Names E1-2.5
Calculated MW 56427 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Shelf Life 18 months from date of despatch.
Gene ID 3783709
Other Names Epstein-Barr nuclear antigen 1, EBNA-1, EBV nuclear antigen 1, EBNA1
Target/Specificity Mouse anti-Epstein-Barr Virus Nuclear Antigen antibody, clone E1-2.5 recognises the repetitive Gly-Ala region of EBV (Epstein-Barr Virus) nuclear antigen 1 (EBNA-1), a viral protein expressed consistently by EBV- associated malignancies.Epstein-Barr Virus, also known as HHV-4 or Human herpesvirus 4, is a member of the herpesvirus family and one of the most common infectious viruses known, being responsible for a wide range of illnesses from infectious mononucleosis to nasal-pharyngeal cancer. EBNA-1 is the only viral protein expressed during group 1 latency and mediates the replication and partitioning of the episome during host cell division, binding to a replication origin (oriP) within the viral genome. The Gly-Ala repeat recognized by Mouse anti-Epstein-Barr Virus Nuclear Antigen antibody, clone E1-2.5, inhibits the host’s CD8-restricted cytotoxic T-cell response to infected cells, by impairing antigen processing and MHC class I-restricted antigen presentation.
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Epstein-Barr Virus Nuclear Antigen Antibody, clone E1-2.5 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. Sim AC et al. (2013) Defining the expression hierarchy of latent T-cell epitopes in Epstein-Barr virus infection with TCR-like antibodies.
Sci Rep. 3: 3232.1. Munz, C. (1999) Epstein-barr virus nuclear antigen 1: from immunologically invisible to a promising T cell target.
J. Exp. Med. 10: 1301-1304.

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Cat# ABD11874
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