|Application ||WB, IHC-P, E, Func|
|Calculated MW||25806 Da|
|Purification||Antisera to rat TNF alpha were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.|
|Immunogen||Recombinant rat TNF alpha (PRP16).|
|Shelf Life||12 months from date of reconstitution.|
|Other Names||Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Tumor necrosis factor, membrane form, N-terminal fragment, NTF, Intracellular domain 1, ICD1, Intracellular domain 2, ICD2, C-domain 1, C-domain 2, Tumor necrosis factor, soluble form, Tnf, Tnfa, Tnfsf2|
|Target/Specificity||Rabbit anti-Rat TNF alpha antibody recognizes rat tumor necrosis factor alpha (TNFα), also known as Cachectin or Tumor necrosis factor ligand superfamily member 2. Rat TNFα is a 235 amino acid ~17 kDa single pass type II O-linked transmembrane glycoprotein, it also exists as a secreted cytokine through cleavage between residues 79 and 80. TNFα is produced by many cell types including macrophages, monocytes, neutrophils and a population of activated NK cells.|
|Preservative & Stabilisers||Preservative Free|
|Storage||Prior to reconstitution store at +4℃. Following reconstitution store at -20℃.|
|Precautions||Anti-Rat TNF alpha Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
1. Halin, S. et al. (2010) Pigment epithelium-derived factor stimulates tumor macrophage recruitment and isdownregulated by the prostate tumor microenvironment.Neoplasia. 12: 336-45. 2. Khandelwal, P.J. et al. (2010) Parkinson-related parkin reduces a-Synuclein phosphorylation in a gene transfer model.Mol Neurodegener. 5: 47. 3. Sandoval, J. et al. (2010) Ordered transcriptional factor recruitment and epigenetic regulation of tnf-alpha in necrotizing acute pancreatitis.Cell Mol Life Sci. 67: 1687-97. 4. Irwin, M.W. et al. (1999) Tissue expression and immunolocalization of tumor necrosis factor-alpha in postinfarction dysfunctional myocardium.Circulation. 99: 1492-8. 5. El-Kharashi, O.A. et al. (2011) The Potential Pharmacological and Histological Benefits of Carvedilol on the Hippocampal Post- Stroke Seizures in RatsLife Science Journal, 8: 951-60 6. Elgarf, A.S. et al. (2014) Lipopolysaccharide repeated challenge followed by chronic mild stress protocol introduces a combined model of depression in rats: reversibility by imipramine and pentoxifylline.Pharmacol Biochem Behav. 126: 152-62. 7. Aharon-Hananel G et al. (2015) Antidiabetic Effect of Interleukin-1β Antibody Therapy Through β-Cell Protection in the Cohen Diabetes-Sensitive Rat.Diabetes. 64 (5): 1780-5.
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.
Request Quote Here