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Anti-Human MCP-1 Antibody

Rabbit Anti-Human Polyclonal Antibody

  • IHC - Anti-Human MCP-1 Antibody  ABD11988
    Staining of formalin fixed, paraffin embedded human breast invasive ductal carcinoma with Rabbit anti Human MCP-1 following heat mediated antigen retrieval using sodium citrate buffer (pH6.0)
  • IHC-P - Anti-Human MCP-1 Antibody  ABD11988
    Staining of formalin fixed, paraffin embedded human breast invasive ductal carcinoma with Rabbit anti Human MCP-1 (AHP784 )following heat mediated antigen retrieval using sodium citrate buffer (pH6.0)
  • E - Anti-Human MCP-1 Antibody  ABD11988
    Sandwich ELISA analysis of human MCP-1 using Mouse anti Human MCP-1 as a capture reagent and biotinylated Rabbit anti Human MCP-1 as a detection reagent with recombinant human MCP-1 as antigen. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader . Serum and plasma samples are included undiluted
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P13500
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 11025 Da
Additional Information
Purification Antisera to human MCP-1 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Immunogen Recombinant human MCP-1 ( PHP061).
Shelf Life 12 months from date of reconstitution.
Gene ID 6347
Other Names C-C motif chemokine 2, HC11, Monocyte chemoattractant protein 1, Monocyte chemotactic and activating factor, MCAF, Monocyte chemotactic protein 1, MCP-1, Monocyte secretory protein JE, Small-inducible cytokine A2, CCL2, MCP1, SCYA2
Target/Specificity Rabbit anti-Human MCP-1 antibody recognizes human MCP-1 (Monocyte Chemoattractant Protein 1), otherwise known as CCL2, a 76 amino acid CC chemokine which acts as a chemoattractant for monocytes, basophils, activated T cells and NK cells, through binding with the seven-transmembrane G protein-coupled receptor, CCR2.The recruitment of mononuclear cells by MCP-1, has established this chemokine as an important factor in innate immunity, implicated in the pathogenesis of inflammatory diseases such as rheumatoid arthritis, multiple sclerosis, uveitis and bronchial asthma. Increasing studies have also linked MCP-1 with the polarization of activated T cells into the T helper 2 (Th2) subset, revealing a role for MCP-1 in the adaptive immune response.Rabbit anti-Human MCP-1 antibody has been successfully used as a detection reagent in the development of a sensitive ELISA assay for the evaluation of MCP-1 expression in conjunction with Mouse anti-Human MCP-1, clone 2.2-4A4-1A11 and recombinant human MCP-1 as a calibration reagent.
Preservative & Stabilisers Preservative Free
Storage Prior to reconstitution store at +4℃. Following reconstitution store at -20℃.
PrecautionsAnti-Human MCP-1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. Banisor, I. et al. (2005) Involvement of beta-chemokines in the development of inflammatory demyelination.
J. Neuroinflammation. 2: 7-20.

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Cat# ABD11988
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