|Calculated MW||144837 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||Mouse spleen cells.|
|Shelf Life||12 months from date of reconstitution.|
|Other Names||Receptor-type tyrosine-protein phosphatase C, 188.8.131.52, Leukocyte common antigen, L-CA, Lymphocyte antigen 5, Ly-5, T200, CD45, Ptprc, Ly-5|
|Target/Specificity||Rat anti-Mouse CD45 antibody, clone YW62.3 recognizes the murine CD45 cell surface antigen, a single pass type1 transmembrane glycoprotein also known as protein tyrosine phosphatase receptor type C (PTPRC) and originally termed Leucocyte Common Antigen (LCA). CD45 is a 180-220kDa glycoprotein expressed by all leucocytes.CD45 is encoded by 3 alleles in mice, differentially expressed by various inbred strains. The Ly5 gene was originally described with the gene product LY5.1 expressed in C57bl/6 and Ly5.2 expressed in SJL strains (Komuraet al.1975), this was subsequently expanded to include a third allele encoding Ly5.3 (Shenet al.1986). Further, in 1987 a reversal of nomenclature was instigated resulting in the allele in C57bl/6 becoming Ly5b encoding Ly5.2 and the allele in SJL mice becoming Ly5a encoding Ly5.1 (Morseet al.1987). Further changes were made in 1992 with Ly5.1 becoming CD45.1 (SJL) and Ly5.2 becoming CD45.2 (C57bl/6). Finally, following work demonstrating homology between the CD45 antigen and a receptor linked protein tyrosine phosphatase the CD45a gene was renamed Ptprca and CD45b renamed Ptprcb(Charbonneauet al.1988;Zebedeeet al.1991).A number of different isoforms isoforms of CD45 are expressed on murine leucocytes depending on the pattern of altenative splicing of 3 exons termed A, B and C encoding regions of ~ 50 amino acids located at the N terminal region of the extracellular portion of CD45. The restricted proteins are termed CD45R with a designation depending on the expressed codon product. (Birkelandet al.1989).Rat anti-mouse CD45 antibody, clone YW62.3 is reactive with all isoforms of murine CD45. N.B. Some reactivity with human tissue has been observed.|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3); 1% Bovine Serum Albumin|
|Storage||Store at +4℃ or -20℃.|
|Precautions||Anti-Mouse CD45 Antibody, clone YW62.3 (FITC) is for research use only and not for use in diagnostic or therapeutic procedures.|
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1. Watt, S.M. et al. (1987) Cell surface markers on haemapoietic precursors. Reagents for the isolation and analysis of progenitor cell subpopulations.Mol. Cell. Probes 1: 297-326. 2. Bindon, C.I. et al. (1988) Importance of antigen specificity for complement-mediated lysis by monoclonal antibodies.Eur. J. Immunol. 18: 1507-1514. 3. Long, G.G. et al. (2010) Hematopoietic Proliferative Lesions in the Spleen of rasH2 Transgenic Mice Treated with MNU.Toxicol Pathol. 38: 1026-36. 4. Drake, C. et al. (2011) Brain inflammation is induced by co-morbidities and risk factors for stroke.Brain Behav Immun. 25: 1113-22. 5. Chan, D.A. et al (2009) Tumor vasculature is regulated by PHD2-mediated angiogenesis and bone marrow-derived cell recruitment.Cancer Cell. 15: 527-38. 6. Lebson, L. et al. (2010) Trafficking CD11b-positive blood cells deliver therapeutic genes to the brain of amyloid-depositing transgenic mice.J Neurosci. 30: 9651-8. 7. Lee, D.C. et al. (2010) LPS- induced inflammation exacerbates phospho-tau pathology in rTg4510 mice.J Neuroinflammation. 7: 56. 8. Wang, S. et al. (2008) Drak2 contributes to West Nile virus entry into the brain and lethal encephalitis.J Immunol. 181: 2084-91. 9. Paz, H. et al. (2010) The homeobox gene Hhex regulates the earliest stages of definitive hematopoiesis.Blood. 116: 1254-62. 10. Reed-Geaghan, E.G. et al. (2010) Deletion of CD14 attenuates Alzheimer's disease pathology by influencing the brain's inflammatory milieu.J Neurosci. 30: 15369-73. 11. Yang, R. et al. (2010) Successful treatment of experimental glomerulonephritis with IdeS and EndoS, IgG-degrading streptococcal enzymes.Nephrol Dial Transplant. 25: 2479-86. 12. Yang, J. et al. (2010) Evaluation of bone marrow- and brain-derived neural stem cells in therapy of central nervous system autoimmunity.Am J Pathol. 177: 1989-2001. 13. Yoshizaki, A. et al. (2010) Cell adhesion molecules regulate fibrotic process via Th1/Th2/Th17 cell balance in a bleomycin-induced scleroderma model.J Immunol. 185: 2502-15. 14. Abramowski, D. et al. (2012) Transgenic Expression of Intraneuronal Aß42 But Not Aß40 Leads to Cellular Aß Lesions, Degeneration, and Functional Impairment without Typical Alzheimer's Disease Pathology.J Neurosci. 32: 1273-83. 15. Dénes, A. et al. (2010) Chronic systemic infection exacerbates ischemic brain damage via a CCL5 (regulated on activation, normal T-cell expressed and secreted)-mediated proinflammatory response in mice.J Neurosci. 30: 10086-95. 16. Kondo, Y. et al. (2007) Osteopetrotic (op/op) mice have reduced microglia, no Abeta deposition, and no changes in dopaminergic neurons.J Neuroinflammation. 4: 31. 17. Lee, S. et al. (2010) CX3CR1 deficiency alters microglial activation and reduces beta-amyloid deposition in two Alzheimer's disease mouse models.Am J Pathol. 177: 2549-62. 18. Kim, J. et al. (2012) Anti-apoE immunotherapy inhibits amyloid accumulation in a transgenic mouse model of Aß amyloidosis.J Exp Med. 209: 2149-56. 19. Yamauchi, S. et al. (2012) Myosin II-dependent exclusion of CD45 from the site of Fcγ receptor activation during phagocytosis.FEBS Lett. 586: 3229-35. 20. Yazid, S. et al. (2015) Annexin-A1 restricts Th17 cells and attenuates the severity of autoimmune disease.J Autoimmun. pii: S0896-8411(14)00183-8. 21. Kan, M.J. et al. (2015) Arginine deprivation and immune suppression in a mouse model of Alzheimer's disease.J Neurosci. 35 (15): 5969-82. 22. Bachstetter, A.D. et al. (2011) Fractalkine and CX 3 CR1 regulate hippocampal neurogenesis in adult and aged rats.Neurobiol Aging. 32 (11): 2030-44. 23. Kuffová, L. et al. (2008) Cross presentation of antigen on MHC class II via the draining lymph node after corneal transplantation in mice.J Immunol. 180 (3): 1353-61. 24. Murinello, S. et al. (2014) Fcγ receptor upregulation is associated with immune complex inflammation in the mouse retina and early age-related macular degeneration.Invest Ophthalmol Vis Sci. 55 (1): 247-58. 24. Zirger, J.M. et al. (2012) Immune-mediated loss of transgene expression from virally transduced brain cells is irreversible, mediated by IFNγ, perforin, and TNFα, and due to the elimination of transduced cells.Mol Ther. 20 (4): 808-19. 25. Mills, J.H. et al. (2012) A2A adenosine receptor signaling in lymphocytes and the central nervous system regulates inflammation during experimental autoimmune encephalomyelitis.J Immunol. 188 (11): 5713-22. 26. McMorran, B.J. et al. (2001) G551D CF mice display an abnormal host response and have impaired clearance of Pseudomonas lung disease.Am J Physiol Lung Cell Mol Physiol. 281 (3): L740-7.
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