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>   home   >   Products   >   Primary Antibodies   >   Stem Cells   >   Anti-Dog CD4 Antibody, clone YKIX302.9 (RPE)   

Anti-Dog CD4 Antibody, clone YKIX302.9 (FITC)

Rat Anti-Dog Monoclonal Antibody

  • FC - Anti-Dog CD4 Antibody, clone YKIX302.9 (FITC) ABD12007
    Staining of canine peripheral blood lymphocytes with Rat anti Canine CD4:APC
  • FC - Anti-Dog CD4 Antibody, clone YKIX302.9 (FITC) ABD12007
    Staining of canine peripheral blood lymphocytes with Rat anti Canine CD4:RPE-Cy7
  • FC - Anti-Dog CD4 Antibody, clone YKIX302.9 (FITC) ABD12007
    Staining of canine peripheral blood lymphocytes with Rat anti Canine CD4:FITC
  • FC - Anti-Dog CD4 Antibody, clone YKIX302.9 (FITC) ABD12007
    Published customer image:Rat anti Dog CD4 antibody, clone YKIX302.9 used for the identification of CD4 expressing dog lymphocytes by flow cytometry.Image caption:Representative dot plots illustrating the analysis of intracellular cytokine profile in T-cell subsets. (A) Pseudocolor plot distribution of short-term in vitro cultured (control or SLA-Ag stimulated) canine whole blood sample according to cell size (Forward scatter - FSC) and granularity (Side scatter- SSC) used for lymphocyte gate selection of FSCLowSSCLow events. (B) Pseudocolor plots representing cytokines?+?(IL-17, TNF-a, IFN-?, TGF-ß and IL-4) CD4+ cells within gated lymphocytes and (C) Pseudocolor plots representing cytokines?+?(IL-17, TNF-a, IFN-?, TGF-ß and IL-4) CD8+ cells within gated lymphocytes. The frequency of cytokines+ T-cells subsets were calculated by quadrant statistics approach and first reported as percentage of gated lymphocytes prior to the calculation of the SLAg/Control indexes.From: Costa-Pereira C, Moreira ML, Soares RP, Marteleto BH, Ribeiro VM, França-Dias MH, Cardoso LM, Viana KF, Giunchetti RC, Martins-Filho OA, Araújo MS. One-year timeline kinetics of cytokine-mediated cellular immunity in dogs vaccinated against visceral leishmaniasis. BMC Vet Res. 2015 Apr 11;11(1):92.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P33705
Reactivity Dog
Host Rat
Clonality Monoclonal
Isotype IgG2a
Clone Names YKIX302.9
Calculated MW 51640 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
Immunogen Canine concanavilin A activated T cell blasts
Shelf Life 18 months from date of despatch.
Gene ID 403931
Other Names T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, CD4, CD4
Target/Specificity Rat anti-Dog CD4 antibody, clone YKIX302.9, is a monoclonal antibody specific for the canine CD4 cell surface antigen. Clone YKIX302.9 was clustered at the first Canine Leukocyte Antigen Workshop (CLAW) [Cobboldet al.1992] along with clone CA13.1E4.Rat anti-Dog CD4 (YKIX302.9) has been demonstrated to partially deplete circulating T lymphocytes when administered in vivo, but alone was not sufficient to prolong allograft survival in a canine transplant model (Watsonet al.1993). Uniquely amongst mammals, canine CD4 is expressed by neutrophils as well as by lymphocytes subsets Mooreet al.1992. Rat anti-Canine CD4 (YKIX302.9) forms part of a panel of anti-canine monoclonal antibodies used extensively in the evaluation of leukemic status in dogs (Villiers 2002).
Preservative & Stabilisers 0.09% Sodium Azide (NaN3); 1% Bovine Serum Albumin
Storage Store at +4℃ or -20℃.
PrecautionsAnti-Dog CD4 Antibody, clone YKIX302.9 (FITC) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. Cobbold, S. & Metcalfe, S. (1994) Monoclonal antibodies that define canine homologues of human CD antigens: summary of the First International Canine Leukocyte Antigen Workshop (CLAW).
Tissue Antigens 43(3): 137-154. 2. Gorman, S. et al. (1994) Isolation and expression of cDNA encoding the canine CD4 and CD8a antigens.
Tissue Antigens 43(3): 184-188. 3. Watson, C.J.E. et al. (1993) CD4 and CD8 monoclonal antibody therapy: strategies to prolong renal allograft survival in the dog.
Brit. J. Surg. 80: 1389-1392. 4. Papadogiannakis, E.I. et al. (2009) Determination of intracellular cytokines IFN-gamma and IL-4 in canine T lymphocytes by flow cytometry following whole-blood culture.
Can J Vet Res. 73: 137-143. 5. Bauer. T.R. Jr. et al. (2006) Correction of the disease phenotype in canine leukocyte adhesion deficiency using ex vivo hematopoietic stem cell gene therapy.
Blood. 108: 3313-20. 6. Boggiatto, P.M. et al. (2010) Immunologic indicators of clinical progression during canine Leishmania infantum infection.
Clin Vaccine Immunol. 17: 267-73. 7. Araújo, M.S. et al. (2011) Immunological changes in canine peripheral blood leukocytes triggered by immunization with first or second generation vaccines against canine visceral leishmaniasis.
Vet Immunol Immunopathol. 141: 64-75. 8. Benyacoub, J. et al. (2003) Supplementation of food with Enterococcus faecium (SF68) stimulates immune functions in young dogs.
J Nutr. 133: 1158-62. 9. Bund, D. et al. (2010) Canine-DCs using different serum-free methods as an approach to provide an animal-model for immunotherapeutic strategies.
Cell Immunol. 263: 88-98. 10. Estrela-Lima, A. et al. (2010) Immunophenotypic features of tumor infiltrating lymphocytes from mammary carcinomas in female dogs associated with prognostic factors and survival rates.
BMC Cancer. 10: 256. 11. Out, T.A. et al. (2002) Local T-cell activation after segmental allergen challenge in the lungs of allergic dogs.
Immunology. 105: 499-508. 12. Pinheiro, D. (2011) Phenotypic and functional characterization of a CD4(+) CD25(high) FOXP3(high) regulatory T-cell population in the dog.
Immunology. 132: 111-22. 13. Mitchell, L. et al. (2012) Clinical and immunomodulatory effects of toceranib combined with low-dose cyclophosphamide in dogs with cancer.
J Vet Intern Med. 26: 355-62. 14. Tominaga, M. et al. (2010) Flow cytometric analysis of peripheral blood and tumor-infiltrating regulatory T cells in dogs with oral malignant melanoma.
J Vet Diagn Invest. 22: 438-41. 15. Figueiredo, M.M. et al. (2014) Expression of Regulatory T Cells in Jejunum, Colon, and Cervical and Mesenteric Lymph Nodes of Dogs Naturally Infected with Leishmania infantum.
Infect Immun. 82: 3704-12. 16. Aresu, L. et al. (2014) VEGF and MMP-9: biomarkers for canine lymphoma.
Vet Comp Oncol. 12: 29-36. 17. Costa-Pereira, C. et al. (2015) One-year timeline kinetics of cytokine-mediated cellular immunity in dogs vaccinated against visceral leishmaniasis.
BMC Vet Res. 11 (1): 92. 18. Hauck, V. et al. (2016) Increased numbers of FoxP3-expressing CD4(+)  CD25(+) regulatory T cells in peripheral blood from dogs with atopic dermatitis and its correlation with disease severity.
Vet Dermatol. 27 (1): 26-e9. 19. Riondato, F. et al. (2015) Analytical and diagnostic validation of a flow cytometric strategy to quantify blood and marrow infiltration in dogs with large b-cell lymphoma.
Cytometry B Clin Cytom. Dec 13. [Epub ahead of print] 20. Miranda, S. et al. (2007) Characterization of circulating lymphocyte subpopulations in canine leishmaniasis throughout treatment with antimonials and allopurinol.
Vet Parasitol. 144 (3-4): 251-60. 21. Yamaya, Y. & Watari, T. (2015) Increased proportions of CCR4(+) cells among peripheral blood CD4(+) cells and serum levels of allergen-specific IgE antibody in canine chronic rhinitis and bronchitis.
J Vet Med Sci. 77 (4): 421-5.

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