|Calculated MW||51640 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.|
|Immunogen||Canine concanavilin A activated T cell blasts|
|Shelf Life||18 months from date of despatch.|
|Other Names||T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, CD4, CD4|
|Target/Specificity||Rat anti-Dog CD4 antibody, clone YKIX302.9, is a monoclonal antibody specific for the canine CD4 cell surface antigen. Clone YKIX302.9 was clustered at the first Canine Leukocyte Antigen Workshop (CLAW) [Cobboldet al.1992] along with clone CA13.1E4.Rat anti-Dog CD4 (YKIX302.9) has been demonstrated to partially deplete circulating T lymphocytes when administered in vivo, but alone was not sufficient to prolong allograft survival in a canine transplant model (Watsonet al.1993). Uniquely amongst mammals, canine CD4 is expressed by neutrophils as well as by lymphocytes subsets Mooreet al.1992. Rat anti-Canine CD4 (YKIX302.9) forms part of a panel of anti-canine monoclonal antibodies used extensively in the evaluation of leukemic status in dogs (Villiers 2002).|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3); 1% Bovine Serum Albumin|
|Storage||Store at +4℃ or -20℃.|
|Precautions||Anti-Dog CD4 Antibody, clone YKIX302.9 (FITC) is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Cobbold, S. & Metcalfe, S. (1994) Monoclonal antibodies that define canine homologues of human CD antigens: summary of the First International Canine Leukocyte Antigen Workshop (CLAW).Tissue Antigens 43(3): 137-154. 2. Gorman, S. et al. (1994) Isolation and expression of cDNA encoding the canine CD4 and CD8a antigens.Tissue Antigens 43(3): 184-188. 3. Watson, C.J.E. et al. (1993) CD4 and CD8 monoclonal antibody therapy: strategies to prolong renal allograft survival in the dog. Brit. J. Surg. 80: 1389-1392. 4. Papadogiannakis, E.I. et al. (2009) Determination of intracellular cytokines IFN-gamma and IL-4 in canine T lymphocytes by flow cytometry following whole-blood culture.Can J Vet Res. 73: 137-143. 5. Bauer. T.R. Jr. et al. (2006) Correction of the disease phenotype in canine leukocyte adhesion deficiency using ex vivo hematopoietic stem cell gene therapy.Blood. 108: 3313-20. 6. Boggiatto, P.M. et al. (2010) Immunologic indicators of clinical progression during canine Leishmania infantum infection.Clin Vaccine Immunol. 17: 267-73. 7. Araújo, M.S. et al. (2011) Immunological changes in canine peripheral blood leukocytes triggered by immunization with first or second generation vaccines against canine visceral leishmaniasis.Vet Immunol Immunopathol. 141: 64-75. 8. Benyacoub, J. et al. (2003) Supplementation of food with Enterococcus faecium (SF68) stimulates immune functions in young dogs.J Nutr. 133: 1158-62. 9. Bund, D. et al. (2010) Canine-DCs using different serum-free methods as an approach to provide an animal-model for immunotherapeutic strategies.Cell Immunol. 263: 88-98. 10. Estrela-Lima, A. et al. (2010) Immunophenotypic features of tumor infiltrating lymphocytes from mammary carcinomas in female dogs associated with prognostic factors and survival rates.BMC Cancer. 10: 256. 11. Out, T.A. et al. (2002) Local T-cell activation after segmental allergen challenge in the lungs of allergic dogs.Immunology. 105: 499-508. 12. Pinheiro, D. (2011) Phenotypic and functional characterization of a CD4(+) CD25(high) FOXP3(high) regulatory T-cell population in the dog.Immunology. 132: 111-22. 13. Mitchell, L. et al. (2012) Clinical and immunomodulatory effects of toceranib combined with low-dose cyclophosphamide in dogs with cancer.J Vet Intern Med. 26: 355-62. 14. Tominaga, M. et al. (2010) Flow cytometric analysis of peripheral blood and tumor-infiltrating regulatory T cells in dogs with oral malignant melanoma.J Vet Diagn Invest. 22: 438-41. 15. Figueiredo, M.M. et al. (2014) Expression of Regulatory T Cells in Jejunum, Colon, and Cervical and Mesenteric Lymph Nodes of Dogs Naturally Infected with Leishmania infantum.Infect Immun. 82: 3704-12. 16. Aresu, L. et al. (2014) VEGF and MMP-9: biomarkers for canine lymphoma.Vet Comp Oncol. 12: 29-36. 17. Costa-Pereira, C. et al. (2015) One-year timeline kinetics of cytokine-mediated cellular immunity in dogs vaccinated against visceral leishmaniasis.BMC Vet Res. 11 (1): 92. 18. Hauck, V. et al. (2016) Increased numbers of FoxP3-expressing CD4(+) CD25(+) regulatory T cells in peripheral blood from dogs with atopic dermatitis and its correlation with disease severity.Vet Dermatol. 27 (1): 26-e9. 19. Riondato, F. et al. (2015) Analytical and diagnostic validation of a flow cytometric strategy to quantify blood and marrow infiltration in dogs with large b-cell lymphoma.Cytometry B Clin Cytom. Dec 13. [Epub ahead of print] 20. Miranda, S. et al. (2007) Characterization of circulating lymphocyte subpopulations in canine leishmaniasis throughout treatment with antimonials and allopurinol.Vet Parasitol. 144 (3-4): 251-60. 21. Yamaya, Y. & Watari, T. (2015) Increased proportions of CCR4(+) cells among peripheral blood CD4(+) cells and serum levels of allergen-specific IgE antibody in canine chronic rhinitis and bronchitis.J Vet Med Sci. 77 (4): 421-5.
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