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>   home   >   Products   >   Primary Antibodies   >   Anti-Pig CD14 Antibody, clone MIL2    

Anti-Pig CD14 Antibody, clone MIL2 (FITC)

Mouse Anti-Pig Monoclonal Antibody

  • FC - Anti-Pig CD14 Antibody, clone MIL2 (FITC) ABD12035
    Staining of pig peripheral blood granulocytes with Mouse anti Pig CD14 followed by Goat anti Mouse IgG:FITC
  • FC - Anti-Pig CD14 Antibody, clone MIL2 (FITC) ABD12035
    Published customer image:Mouse anti Pig CD14 antibody, clone MIL2 used for the evaluation of CD14 expression on porcine leukocytes by flow cytometry.Image caption:Overview of flow cytometry analyses. (A) Gating strategy for identification of monocytes and macrophages. The peripheral blood is shown. Designation of population shown within each dot-plot is indicated above the dot-plot. Leukocytes were identified as viable (a) non-doublet (b) cells with typical light scatter properties of leukocytes (c). Then, macrophages were gated simply as CD203ahi leukocytes (d) and marked with blue color. Monocytes were gated as CD203alow/-SWC8-(e) CD172ahi(f) leukocytes where the CD203alow/- region was defined as the complementary region to the CD203ahi region. Then, SLA-DR+ monocytes were marked with red color and SLA-DR- monocytes were marked with green color (g). SLA-DR- region was defined as the complementary region to the SLA-DR+ region. Gating order is shown in the scheme (h). (B) Representative CD163 vs. CD14 dot-plots of macrophages and monocyte subpopulations (green: SLA-DR–, red: SLA-DR+) in various body compartments of control and APP-infected pigs.From: Ondrackova P, Leva L, Kucerova Z, Vicenova M, Mensikova M, Faldyna M. Distribution of porcine monocytes in different lymphoid tissues and the lungs during experimental Actinobacillus pleuropneumoniae infection and the role of chemokines.Vet Res. 2013 Oct 17;44:98.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Reactivity Pig
Host Mouse
Clonality Monoclonal
Isotype IgG2b
Clone Names MIL2
Additional Information
Other Species H
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen Porcine peripheral blood lymphocytes.
Shelf Life 18 months from date of despatch.
Target/Specificity Mouse anti-Pig CD14, clone MIL2 recognizes porcine CD14. Clone MIL2 was clustered as porcine CD14 at the Third International Workshop on Swine Leukocyte Differentiation Antigens (Haversonet al.2001) . Clone MIL2 immunoprecipitates a protein of ~50 kDa consistent with the expected apparent molecular weight of porcine CD14, and demonstrates the expected CD14 profile by dual labelling and competition studies. Further, pre-incubation of peripheral blood monocytes with MIL2 inhibits the binding of FITC labelled LPS, consistent with masking the CD14 LPS binding site (Thackeret al.2001) . Mouse anti-pig CD14, clone MIL2 demonstrates staining of both monocytes and neutrophils in peripheral blood by flow cytometry with a similar expression pattern to the anti-human CD14 clone TüK4, lymphocytes and eosinophils are negative for MIL2 staining (Zelnickovaet al.2007). Cloning and characterization of porcine CD14 indicates a high degree of both functional and structural conservation when compared to CD14 from other mammalian species, the gene maps to chromosome 2 and is expressed on a wide range of tissues in a manner consistent with expression on myeloid cells. (Petersenet al.2007,Sanzet al.2007).
Preservative & Stabilisers 0.09% Sodium Azide (NaN3); 1% Bovine Serum Albumin
Storage Store at +4℃ or -20℃.
PrecautionsAnti-Pig CD14 Antibody, clone MIL2 (FITC) is for research use only and not for use in diagnostic or therapeutic procedures.
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1. Haverson, K. et al. (1994) Characterization of monoclonal antibodies specific for monocytes, macrophages and granulocytes from porcine peripheral blood and mucosal tissues.
J Immunol Methods. 170 (2): 233-45. 2. Thacker, E. et al. (2001) Summary of workshop findings for porcine myelomonocytic markers.
Vet Immunol Immunopathol. 80 (1-2): 93-109. 3. Thorgersen, E.B. et al. (2010) CD14 inhibition efficiently attenuates early inflammatory and hemostatic responses in Escherichia coli sepsis in pigs.
FASEB J. 24: 712-22. 4. Goujon, J.M. et al. (2000) Influence of cold-storage conditions on renal function of autotransplanted large pig kidneys.
Kidney Int. 58: 838-50. 5. Li, Y. et al. (2014) Identification of apoptotic cells in the thymus of piglets infected with highly pathogenic porcine reproductive and respiratory syndrome virus.
Virus Res. pii: S0168-1702(14)00173-7. 6. Summerfield, A. et al. (2003) Porcine peripheral blood dendritic cells and natural interferon-producing cells.
Immunology. 110: 440-9. 7. Vanderheijden, N. et al. (2003) Involvement of sialoadhesin in entry of porcine reproductive and respiratory syndrome virus into porcine alveolar macrophages.
J Virol. 77: 8207-15. 8. Barratt-Due, A. et al.(2011)Ornithodoros moubata Complement Inhibitor Is an Equally Effective C5 Inhibitor in Pigs and Humans.
J Immunol. 187: 4913-9. 9. Hauet, T. et al. (2002) Polyethylene glycol reduces the inflammatory injury due to cold ischemia/reperfusion in autotransplanted pig kidneys.
Kidney Int. 62: 654-67. 10. Kapetanovic, R. et al. (2012) Pig bone marrow-derived macrophages resemble human macrophages in their response to bacterial lipopolysaccharide.
J Immunol. 188: 3382-94. 11. Thorgersen, E.B. et al. (2009) Inhibition of complement and CD14 attenuates the Escherichia coli-induced inflammatory response in porcine whole blood.
Infect Immun. 77: 725-32. 12. Zelnickova, P. et al. (2007) Intracellular cytokine detection by flow cytometry in pigs: fixation, permeabilization and cell surface staining.
J Immunol Methods. 327: 18-29. 13. Facci, M.R. et al. (2011) Stability of expression of reference genes in porcine peripheral blood mononuclear and dendritic cells.
Vet Immunol Immunopathol. 141: 11-5. 14. Koutná, I. et al. (2012) Flow Cytometry Analysis of Intracellular Protein
In: Flow Cytometry - Recent Perspectives, Schmid, I. (Ed.), ISBN: 978-953-51- 15. Facci, M.R. et al. (2010) A comparison between isolated blood dendritic cells and monocyte-derived dendritic cells in pigs.
Immunology. 129: 396-405. 16. Schierack, P. et al. (2009) Effects of Bacillus cereusvar.toyoi on immune parameters of pregnant sows.
Vet Immunol Immunopathol.127: 26-37. 17. Lundeland, B. et al. (2011) Severe gunshot injuries in a porcine model: impact on central markers of innate immunity.
Acta Anaesthesiol Scand. 55: 28-34. 18. Thorgersen, E.B. et al. (2008) Cyanobacterial LPS antagonist (CyP)-a novel and efficient inhibitor of Escherichia coli LPS-induced cytokine response in the pig.
Mol Immunol. 45: 3553-7. 19. Schierack, P. et al.(2007)Bacillus cereusvar.toyoi enhanced systemic immune response in piglets.
Vet Immunol Immunopathol. 118: 1-11. 20. Ondrackova, P. et al. (2012) Interaction of porcine neutrophils with different strains of enterotoxigenic Escherichia coli.
Vet Microbiol. 160: 108-16. 21. Ondrackova, P. et al. (2012) Phenotypic characterisation of the monocyte subpopulations in healthy adult pigs and Salmonella-infected piglets by seven-colour flow cytometry.
Res Vet Sci. pii: S0034-5288(12)00256-1. 22. Vicenova, M. et al. (2014) Evaluation of in vitroandin vivo anti-inflammatory activity of biologically active phospholipids with anti-neoplastic potential in porcine model.
BMC Complement Altern Med. 14: 339. 23. Alvarez, B. et al. (2015) Phenotypic and functional heterogeneity of CD169+ and CD163+ macrophages from porcine lymph nodes and spleen.
Dev Comp Immunol. 44: 44-9. 27. Egge, K.H. et al. (2015) Organ inflammation in porcine Escherichia coli sepsis is markedly attenuated by combined inhibition of C5 and CD14.
Immunobiology. 220 (8): 999-1005. 28. Moffat, L. et al. (2014) Development and characterisation of monoclonal antibodies reactive with porcine CSF1R (CD115).
Dev Comp Immunol. 47 (1): 123-8. 29. Nguyen DN et al. (2015) Oral antibiotics increase blood neutrophil maturation and reduce bacteremia and necrotizing enterocolitis in the immediate postnatal period of preterm pigs.
Innate Immun. pii: 1753425915615195. 30. Liu J et al. (2016) The Role of Porcine Monocyte Derived Dendritic Cells (MoDC) in the Inflammation Storm Caused by Streptococcus suis Serotype 2 Infection.
PLoS One. 11 (3): e0151256.1. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.2. Petersen, C.B. et al. (2007) Cloning, characterization and mapping of porcine CD14 reveals a high conservation of mammalian CD14 structure, expression and locus organization.
Dev Comp Immunol. 31: 729-37.3. Sanz, G. et al. (2007) Molecular cloning, chromosomal location, and expression analysis of porcine CD14.
Dev Comp Immunol. 2007;31(7):738-47.

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