|Application ||IHC-F, FC, IP|
|Calculated MW||42288 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G.|
|Immunogen||Mouse B cell lymphoma, 38C-14.|
|Shelf Life||18 months from date of despatch.|
|Other Names||L-selectin, CD62 antigen-like family member L, Leukocyte adhesion molecule 1, LAM-1, Leukocyte-endothelial cell adhesion molecule 1, LECAM1, Lymph node homing receptor, Lymphocyte antigen 22, Ly-22, Lymphocyte surface MEL-14 antigen, CD62L, Sell, Lnhr, Ly-22, Ly22|
|Target/Specificity||Rat anti-mouse CD62L, clone MEL-14, recognizesmouse L-selectin. L-selectin is an adhesion molecule expressed on most T and B lymphocytes, neutrophils, monocytes and eosinophils, (Fink, P. et al., 1985).Pre-incubation of lymphocytes with MEL-14 completely and specifically blocks binding of lymphocytes to high endothelial venules (HEV) in vitro and the migration of lymphocytes to lymph nodes in vivo(Gallatin, W. et al. 1983). Removal of sodium azide is recommended prior to using this clone in functional assays. AbD Serotec recommend a Slide-A-Lyzer® Dialysis Cassette for this purpose.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Mouse CD62L Antibody, clone MEL-14 is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Fink, P. et al. (1985) Homing receptor-bearing thymocytes, an immunocompetent cortical subpopulationNature 313: 233-235. 2. Gallatin, W.M. et al. (1983) A cell surface molecule involved in organ specific homing of lymphocytes.Nature 304: 30-34. 3. Lewinsohn, D.M. et al. (1987) Leukocyte-endothelial cell recognition: evidence of a common molecular mechanism shared by neutrophils, lymphocytes, and other leukocytes.J. Immunol. 138: 4313-4321. 4. Reichert, R.M. et al. (1984) A homing receptor-bearing cortical thymocyte subset: Implications for thymus cell migration and the nature of cortisone-resistant thymocytes.Cell 38: 89-99. 5. Siegleman, M. et al. (1990) The mouse lymph node homing receptor is identical with the lymphocyte cell surface marker Ly-22: role of the EGF domain in endothelial bindingCell 61: 611-622. 6. Jalkanen, S. et al. (1987) Lymphocyte recognition of high endothelium: antibodies to distinct epitopes of an 85-95-kD glycoprotein antigen differentially inhibit lymphocyte binding to lymph node, mucosal, or synovial endothelial cells. J. Cell. Biol. 105: 983 - 990.
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