|Application ||IHC-F, FC, IP, Func|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||B6.1 CTL cell line|
|Shelf Life||18 months from date of despatch.|
|Other Names||Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, p55, CD25, Il2ra, Il2r|
|Target/Specificity||Rat anti-Mouse CD25 antibody, clone PC61.5.3 reacts with the low affinity alpha chain of the interleukin-2 receptor present on activated T and B cells in mice. Rat anti-Mouse CD25 antibody, clone PC61.5.3 is reported to inhibit IL-2 binding and IL-2 dependent proliferation.|
|Preservative & Stabilisers||Preservative Free|
|Storage||Store at -20℃ only.|
|Precautions||Anti-Mouse CD25 Antibody, clone PC61.5.3 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Moreau, J.L. et al. (1987) Monoclonal antibodies identify three epitope clusters on the mouse p55 subunit of the interleukin 2 receptor: relationship to the interleukin 2-binding site.Eur J Immunol. 17 (7): 929-35. 2. Hashimoto, N. et al. (1986) Dissociation of interleukin 2-dependent and -independent B cell proliferation with monoclonal anti-interleukin 2 receptor antibody.Eur J Immunol. 16 (3): 317-20. 3. Lowenthal, J.W. et al. (1985) High and low affinity IL 2 receptors: analysis by IL 2 dissociation rate and reactivity with monoclonal anti-receptor antibody PC61.J Immunol. 135 (6): 3988-94. 4. Ceredig, R. et al. (1985) Expression of interleukin-2 receptor as a differentiation marker on intrathymic stem cells.Nature 314: 98-100. 5. Yaqoob, P. and Calder, P.C. (1997) Glutamine Requirement of Proliferating T lymphocytes.Nutrition 13: 646-651. 6. Scotland, R.S. et al. (2011) Sex differences in resident immune cell phenotype underlie more efficient acute inflammatory responses in female mice.Blood. 118 (22): 5918-27.
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