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Anti-Rat Interferon gamma Antibody, clone DB-1

Mouse Anti-Rat Monoclonal Antibody

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P01581
Reactivity Rat
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names DB-1
Calculated MW 17918 Da
Additional Information
Other Species M
Purification Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Immunogen Recombinant rat IFN gamma
Shelf Life 18 months from date of despatch.
Gene ID 25712
Other Names Interferon gamma, IFN-gamma, Ifng
Target/Specificity Mouse anti-Rat Interferon gamma antibody, clone DB-1 reacts with both mouse and rat Interferon gamma. Clone DB-1 exhibits high affinity binding to the natural and recombinant forms and effectively neutralizes the anti-viral activity of IFN-gamma. It also blocks the induction of Ia antigen expression on murine keratinocytes.Mouse anti-Rat Interferon gamma antibody, clone DB-1 does not cross-react with rodent IFN-alpha or IFN-beta or with human interferons.Mouse anti-Rat Interferon gamma antibody, clone DB-1 has also been reported for use in neutralising studies.
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Rat Interferon gamma Antibody, clone DB-1 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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1. Paineau, J. et al. (1991) Effect of recombinant interferon gamma and interleukin-2 and of a monoclonal antibody against interferon gamma on the rat immune response against heart allografts.
J. Heart Lung Transplant. 10: 424-430. 2. Paineau, J. et al.. (1989) Effects of gamma interferon and interleukin-2, and of gamma-interferon antibodies, on the rat immune response against allografts.
Transplant Proc. 21: 999-1001. 3. Van der Meide, P.H. et al. (1986) The purification and characterization of rat gamma interferon by use of two monoclonal antibodies.
J. Gen. Virol. 67: 1059-1071. 4. Keller, R. et al. (1987) Induction, maintenance, and reinduction of tumoricidal activity in bone marrow-derived mononuclear phagocytes by Corynebacterium parvum. Evidence for the involvement of a T cell- and interferon-gamma-independent pathway of macrophage activation.
J. Immunol. 138: 2366-12371. 5. Jacob, C.O. et al. (1989) Heterogeneous effects of IFN-gamma in adjuvant arthritis.
J. Immunol. 142: 1500-1505. 6. Kingston, A.E. et al. (1989) Schwann cells co-cultured with stimulated T cells and antigen express major histocompatibility complex (MHC) class II determinants without interferon-gamma pretreatment: synergistic effects of interferon-gamma and tumor necrosis factor on MHC class II induction.
Eur. J. Immunol. 19: 177-183. 7. Graf, M. et al. (2001) IL-6 secretion by a rat T9 Glioma clone induces a neutrophil-dependent antitumor response with resultant cellular, antiglioma immunity.
J. Immunol. 166: 121 - 129. 8. Elflein, K. et al. (2003) Rapid recovery from T lymphopenia by CD28 superagonist therapy.
Blood. 102: 1764-70. 9. Straube, F. and Herrmann, T. (2001) Differential modulation of CD8beta by rat gammadelta and alphabeta T cells after activation.
Immunology. 104: 252-8. 10. van der Meide PH et al. (1989) Isolation and characterization of monoclonal antibodies directed to rat interferon-gamma.
Lymphokine Res. 8 (4): 439-49.

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Cat# ABD12052
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